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A retinoblastoma orthologue is a major regulator of S-phase, mitotic, and developmental gene expression in Dictyostelium.

Author(s): Strasser K, Bloomfield G, MacWilliams A, Ceccarelli A, MacWilliams H, Tsang A

PLoS One. 2012;7(6):e39914 Authors: Strasser K, Bloomfield G, MacWilliams A, Ceccarelli A, MacWilliams H, Tsang A

Article GUID: 22768168
mycoCLAP, the database for characterized lignocellulose-active proteins of fungal origin: resource and text mining curation support.

Author(s): Strasser K, McDonnell E, Nyaga C, Wu M, Wu S, Almeida H, Meurs MJ, Kosseim L, Powlowski J, Butler G, Tsang A

Database (Oxford). 2015;2015: Authors: Strasser K, McDonnell E, Nyaga C, Wu M, Wu S, Almeida H, Meurs MJ, Kosseim L, Powlowski J, Butler G, Tsang A

Article GUID: 25754864
Manual Gene Curation and Functional Annotation.

Author(s): McDonnell E, Strasser K, Tsang A

Methods Mol Biol. 2018;1775:185-208 Authors: McDonnell E, Strasser K, Tsang A

Article GUID: 29876819
Investigation of inter- and intraspecies variation through genome sequencing of Aspergillus section Nigri.

Author(s): Vesth TC, Nybo JL, Theobald S, Frisvad JC, Larsen TO, Nielsen KF, Hoof JB, Brandl J, Salamov A, Riley R, Gladden JM, Phatale P, Nielsen MT, ...

Nat Genet. 2018 12;50(12):1688-1695 Authors: Vesth TC, Nybo JL, Theobald S, Frisvad JC, Larsen TO, Nielsen KF, Hoof JB, Brandl J, Salamov A, Riley R, Gladden JM, Phatale P, Nielsen MT, Lyhne EK, K...

Article GUID: 30349117
The gold-standard genome of Aspergillus niger NRRL 3 enables a detailed view of the diversity of sugar catabolism in fungi.

Author(s): Aguilar-Pontes MV, Brandl J, McDonnell E, Strasser K, Nguyen TTM, Riley R, Mondo S, Salamov A, Nybo JL, Vesth TC, Grigoriev IV, Andersen MR,...

Stud Mycol. 2018 Sep;91:61-78 Authors: Aguilar-Pontes MV, Brandl J, McDonnell E, Strasser K, Nguyen TTM, Riley R, Mondo S, Salamov A, Nybo JL, Vesth TC, Grigoriev IV, Andersen MR, Tsang A, de Vrie...

Article GUID: 30425417
Title:Manual Gene Curation and Functional Annotation.
Authors:McDonnell E,  Strasser K,  Tsang A
Link:https://www.ncbi.nlm.nih.gov/pubmed/29876819?dopt=Abstract
DOI:10.1007/978-1-4939-7804-5_16
Category:Methods Mol Biol
PMID:29876819
Dept Affiliation: GENOMICS
1 Centre for Structural and Functional Genomics, Concordia University, Montreal, QC, Canada.
2 Centre for Structural and Functional Genomics, Concordia University, Montreal, QC, Canada. kimchi.strasser@concordia.ca.

Description:

Manual Gene Curation and Functional Annotation.

Methods Mol Biol. 2018;1775:185-208

Authors: McDonnell E, Strasser K, Tsang A

Abstract

No genome sequencing project is complete without structural and functional annotation. Gene models and functional predictions for these models can be obtained relatively easily using computational methods, but they are prone to errors. We describe herein the steps we use to manually curate gene models and functionally annotate them. Our approach is to examine each gene model carefully, and improve its structure if necessary, using a comprehensive set of experimental and computational data as evidence. Then, functional predictions are assigned to the gene models based on conserved protein domains and sequence similarities. We use stringent sequence similarity cutoffs and reviewed sequence-database records as external sources for our annotations. By methodically choosing which evidence to use for each annotation, we minimize the risk of adopting and assigning false predictions to the gene models.

PMID: 29876819 [PubMed - indexed for MEDLINE]