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Biochemical and molecular characterization of a hydroxyjasmonate sulfotransferase from Arabidopsis thaliana.

Author(s): Gidda SK, Miersch O, Levitin A, Schmidt J, Wasternack C, Varin L

J Biol Chem. 2003 May 16;278(20):17895-900 Authors: Gidda SK, Miersch O, Levitin A, Schmidt J, Wasternack C, Varin L

Article GUID: 12637544
Biochemical and molecular characterization of a cellobiohydrolase from Trametes versicolor.

Author(s): Lahjouji K, Storms R, Xiao Z, Joung KB, Zheng Y, Powlowski J, Tsang A, Varin L

Appl Microbiol Biotechnol. 2007 May;75(2):337-46 Authors: Lahjouji K, Storms R, Xiao Z, Joung KB, Zheng Y, Powlowski J, Tsang A, Varin L

Article GUID: 17333176
RNA-Based Therapy Utilizing Oculopharyngeal Muscular Dystrophy Transcript Knockdown and Replacement.

Author(s): Abu-Baker A, Kharma N, Perreault J, Grant A, Shekarabi M, Maios C, Dona M, Neri C, Dion PA, Parker A, Varin L, Rouleau GA

Mol Ther Nucleic Acids. 2019 Apr 15;15:12-25 Authors: Abu-Baker A, Kharma N, Perreault J, Grant A, Shekarabi M, Maios C, Dona M, Neri C, Dion PA, Parker A, Varin L, Rouleau GA

Article GUID: 30831428
Title:Biochemical and molecular characterization of a cellobiohydrolase from Trametes versicolor.
Authors:Lahjouji K,  Storms R,  Xiao Z,  Joung KB,  Zheng Y,  Powlowski J,  Tsang A,  Varin L
Link:https://www.ncbi.nlm.nih.gov/pubmed/17333176?dopt=Abstract
DOI:10.1007/s00253-006-0824-5
Category:Appl Microbiol Biotechnol
PMID:17333176
Dept Affiliation: BIOLOGY
1 Centre for Structural and Functional Genomics, Biology Department, Concordia University, 7141 Sherbrooke street West, Montréal, Quebec, H4B 1R6, Canada.

Description:

Biochemical and molecular characterization of a cellobiohydrolase from Trametes versicolor.

Appl Microbiol Biotechnol. 2007 May;75(2):337-46

Authors: Lahjouji K, Storms R, Xiao Z, Joung KB, Zheng Y, Powlowski J, Tsang A, Varin L

Abstract

A cellobiohydrolase-encoding cDNA, Tvcel7a, from Trametes versicolor has been cloned and expressed in Aspergillus niger. The deduced amino acid sequence shows that Tvcel7a encodes a 456-amino acid polypeptide belonging to glycosyl hydrolase family 7. TvCel7a possesses a 19-amino acid secretion signal but does not possess a linker region nor a carbohydrate-binding domain. Two peaks of activity were obtained after TvCel7a was purified to apparent homogeneity by gel-filtration followed by anion-exchange chromatography. Mass spectrometry performed on the purified proteins confirmed that both peaks corresponded to the predicted sequence of the T. versicolor cellulase. The biochemical properties of the purified TvCel7a obtained from both peaks were studied in detail. The pH and temperature optima were 5.0 and 40 degrees C, respectively. The enzyme was stable over a pH range extending from pH 3.0 to 9.0 and at temperatures lower than 50 degrees C. The kinetic parameters with the substrate p-nitrophenyl beta-D: -cellobioside (pNPC) were 0.58 mM and 1.0 micromol/min/mg protein for the purified TvCel7a found in both peaks 1 and 2. TvCel7a catalyzes the hydrolysis of pNPC, filter paper, beta-glucan, and avicel to varying extents, but no detectable hydrolysis was observed when using the substrates carboxymethylcellulose, laminarin and pNPG.

PMID: 17333176 [PubMed - indexed for MEDLINE]