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Closely related fungi employ diverse enzymatic strategies to degrade plant biomass.

Author(s): Benoit I, Culleton H, Zhou M, DiFalco M, Aguilar-Osorio G, Battaglia E, Bouzid O, Brouwer CPJM, El-Bushari HBO, Coutinho PM, Gruben BS, Hild...

Biotechnol Biofuels. 2015;8:107 Authors: Benoit I, Culleton H, Zhou M, DiFalco M, Aguilar-Osorio G, Battaglia E, Bouzid O, Brouwer CPJM, El-Bushari HBO, Coutinho PM, Gruben BS, Hildén KS, Hou...

Article GUID: 26236396
Secretion of small proteins is species-specific within Aspergillus sp.

Author(s): Valette N, Benoit-Gelber I, Falco MD, Wiebenga A, de Vries RP, Gelhaye E, Morel-Rouhier M

Microb Biotechnol. 2017 03;10(2):323-329 Authors: Valette N, Benoit-Gelber I, Falco MD, Wiebenga A, de Vries RP, Gelhaye E, Morel-Rouhier M

Article GUID: 27153937
Evolutionary Adaptation to Generate Mutants.

Author(s): de Vries RP, Lubbers R, Patyshakuliyeva A, Wiebenga A, Benoit-Gelber I

Methods Mol Biol. 2018;1775:133-137 Authors: de Vries RP, Lubbers R, Patyshakuliyeva A, Wiebenga A, Benoit-Gelber I

Article GUID: 29876815
Investigation of inter- and intraspecies variation through genome sequencing of Aspergillus section Nigri.

Author(s): Vesth TC, Nybo JL, Theobald S, Frisvad JC, Larsen TO, Nielsen KF, Hoof JB, Brandl J, Salamov A, Riley R, Gladden JM, Phatale P, Nielsen MT, ...

Nat Genet. 2018 12;50(12):1688-1695 Authors: Vesth TC, Nybo JL, Theobald S, Frisvad JC, Larsen TO, Nielsen KF, Hoof JB, Brandl J, Salamov A, Riley R, Gladden JM, Phatale P, Nielsen MT, Lyhne EK, K...

Article GUID: 30349117
Title:Evolutionary Adaptation to Generate Mutants.
Authors:de Vries RP,  Lubbers R,  Patyshakuliyeva A,  Wiebenga A,  Benoit-Gelber I
Link:https://www.ncbi.nlm.nih.gov/pubmed/29876815?dopt=Abstract
DOI:10.1007/978-1-4939-7804-5_12
Category:Methods Mol Biol
PMID:29876815
Dept Affiliation: BIOLOGY
1 Fungal Physiology, Westerdijk Fungal Biodiversity Institute, Utrecht, The Netherlands. r.devries@westerdijkinstitute.nl.
2 Fungal Physiology, Westerdijk Fungal Biodiversity Institute, Utrecht, The Netherlands.
3 Centre for Structural and Functional Genomics, Department of Biology, Concordia University, Montreal, QC, Canada.

Description:

Evolutionary Adaptation to Generate Mutants.

Methods Mol Biol. 2018;1775:133-137

Authors: de Vries RP, Lubbers R, Patyshakuliyeva A, Wiebenga A, Benoit-Gelber I

Abstract

In this chapter we describe a method to generate mutants of filamentous fungi using their genomic plasticity and rapid adaptability to their environment. This method is based on spontaneous mutations occurring in relation to improved growth of fungi on media by repeated inoculation resulting in adaptation of the strain to the condition. The critical aspect of this method is the design of the selective media, which will depend strongly on the phenomenon that will be studied. This method is advantageous over UV or chemical random mutagenesis as it results in a lower frequency of undesired mutations and can result in strains that combined with (post)genomic approaches can enhance our understanding of the mechanisms driving various biological processes. In addition, it can be used to obtain better strains for various industrial applications. The method described here is specific for sporulating fungi and has so far not yet been tested for nonsporulating fungi.

PMID: 29876815 [PubMed - indexed for MEDLINE]