Keyword search (3,448 papers available)


A Highly Characterized Synthetic Landing Pad System for Precise Multicopy Gene Integration in Yeast.

Author(s): Bourgeois L, Pyne ME, Martin VJJ

ACS Synth Biol. 2018 Nov 16;7(11):2675-2685 Authors: Bourgeois L, Pyne ME, Martin VJJ

Article GUID: 30372609
Engineering of a Nepetalactol-Producing Platform Strain of Saccharomyces cerevisiae for the Production of Plant Seco-Iridoids.

Author(s): Campbell A, Bauchart P, Gold ND, Zhu Y, De Luca V, Martin VJ

ACS Synth Biol. 2016 05 20;5(5):405-14 Authors: Campbell A, Bauchart P, Gold ND, Zhu Y, De Luca V, Martin VJ

Article GUID: 26981892
Mining Enzyme Diversity of Transcriptome Libraries through DNA Synthesis for Benzylisoquinoline Alkaloid Pathway Optimization in Yeast.

Author(s): Narcross L, Bourgeois L, Fossati E, Burton E, Martin VJ

ACS Synth Biol. 2016 12 16;5(12):1505-1518 Authors: Narcross L, Bourgeois L, Fossati E, Burton E, Martin VJ

Article GUID: 27442619
A Combinatorial Approach To Study Cytochrome P450 Enzymes for De Novo Production of Steviol Glucosides in Baker's Yeast.

Author(s): Gold ND, Fossati E, Hansen CC, DiFalco M, Douchin V, Martin VJJ

ACS Synth Biol. 2018 Dec 21;7(12):2918-2929 Authors: Gold ND, Fossati E, Hansen CC, DiFalco M, Douchin V, Martin VJJ

Article GUID: 30474973
An Automated Induction Microfluidics System for Synthetic Biology.

Author(s): Husser MC, Vo PQN, Sinha H, Ahmadi F, Shih SCC

ACS Synth Biol. 2018 03 16;7(3):933-944 Authors: Husser MC, Vo PQN, Sinha H, Ahmadi F, Shih SCC

Article GUID: 29516725
Title:Engineering of a Nepetalactol-Producing Platform Strain of Saccharomyces cerevisiae for the Production of Plant Seco-Iridoids.
Authors:Campbell A,  Bauchart P,  Gold ND,  Zhu Y,  De Luca V,  Martin VJ
Link:https://www.ncbi.nlm.nih.gov/pubmed/26981892?dopt=Abstract
DOI:10.1021/acssynbio.5b00289
Category:ACS Synth Biol
PMID:26981892
Dept Affiliation: GENOMICS
1 Department of Biology, Centre for Structural and Functional Genomics, Concordia University , Montréal, Québec H4B 1R6, Canada.
2 Department of Biological Sciences, Brock University , 1812 Sir Isaac Brock Way, St. Catharines, Ontario L2S 3A1, Canada.

Description:

Engineering of a Nepetalactol-Producing Platform Strain of Saccharomyces cerevisiae for the Production of Plant Seco-Iridoids.

ACS Synth Biol. 2016 05 20;5(5):405-14

Authors: Campbell A, Bauchart P, Gold ND, Zhu Y, De Luca V, Martin VJ

Abstract

The monoterpene indole alkaloids (MIAs) are a valuable family of chemicals that include the anticancer drugs vinblastine and vincristine. These compounds are of global significance-appearing on the World Health Organization's list of model essential medicines-but remain exorbitantly priced due to low in planta levels. Chemical synthesis and genetic manipulation of MIA producing plants such as Catharanthus roseus have so far failed to find a solution to this problem. Synthetic biology holds a potential answer, by building the pathway into more tractable organisms such as Saccharomyces cerevisiae. Recent work has taken the first steps in this direction by producing small amounts of the intermediate strictosidine in yeast. In order to help improve on these titers, we aimed to optimize the early biosynthetic steps of the MIA pathway to the metabolite nepetalactol. We combined a number of strategies to create a base strain producing 11.4 mg/L of the precursor geraniol. We also show production of the critical intermediate 10-hydroxygeraniol and demonstrate nepetalactol production in vitro. Lastly we demonstrate that activity of the iridoid synthase toward the intermediates geraniol and 10-hydroxygeraniol results in the synthesis of the nonproductive intermediates citronellol and 10-hydroxycitronellol. This discovery has serious implications for the reconstruction of the MIA in heterologous organisms.

PMID: 26981892 [PubMed - indexed for MEDLINE]