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The binding of Na(+) to apo-enolase permits the binding of substrate.

Author(s): Lin T, Kornblatt MJ

Biochim Biophys Acta. 2000 Feb 09;1476(2):279-86 Authors: Lin T, Kornblatt MJ

Article GUID: 10669792
Cloning, expression and mutagenesis of a subunit contact of rabbit muscle-specific (betabeta) enolase.

Author(s): Kornblatt MJ, Zheng SX, Lamandé N, Lazar M

Biochim Biophys Acta. 2002 Jun 03;1597(2):311-9 Authors: Kornblatt MJ, Zheng SX, Lamandé N, Lazar M

Article GUID: 12044909
Translational regulation in chloroplasts for development and homeostasis.

Author(s): Sun Y, Zerges W

Biochim Biophys Acta. 2015 Sep;1847(9):809-20 Authors: Sun Y, Zerges W

Article GUID: 25988717
Title:Cloning, expression and mutagenesis of a subunit contact of rabbit muscle-specific (betabeta) enolase.
Authors:Kornblatt MJ,  Zheng SX,  Lamandé N,  Lazar M
Link:https://www.ncbi.nlm.nih.gov/pubmed/12044909?dopt=Abstract
DOI:10.1016/s0167-4838(02)00319-9
Category:Biochim Biophys Acta
PMID:12044909
Dept Affiliation: CHEMBIOCHEM
1 Enzyme Research Group, Department of Chemistry and Biochemistry, Concordia University, 1455 de Maisonneuve Boulevard W., Montreal, Quebec, Canada H3G 1M8. judithk@vax2.concordia.ca

Description:

Cloning, expression and mutagenesis of a subunit contact of rabbit muscle-specific (betabeta) enolase.

Biochim Biophys Acta. 2002 Jun 03;1597(2):311-9

Authors: Kornblatt MJ, Zheng SX, Lamandé N, Lazar M

Abstract

The cDNA for rabbit muscle-specific (betabeta) enolase was cloned, sequenced and expressed in Escherichia coli. This betabeta-enolase differs at eight positions from that sequenced by Chin (17). Site-directed mutagenesis was used to change residue 414 from glutamate to leucine, thereby abolishing a salt bridge involved in subunit contacts. Recombinant wild-type and mutant enolase were purified from E. coli and compared to enolase isolated from rabbit muscle. Molecular weights were determined by mass spectrometry. All three betabeta-enolases had similar kinetics, and UV and circular dichroism (CD) spectra. The mutant enolase was far more sensitive to inactivation by pressure, by KCl or EDTA, and by sodium perchlorate. We confirmed, by analytical ultracentrifugation, that the sodium perchlorate inactivation was due to dissociation. DeltaG(o) for dissociation of enolase was decreased from 49.7 kJ/mol for the wild-type enzyme to 42.3 kJ/mol for the mutant. In contrast to the wild-type enzyme, perchlorate inactivation of E414L was accompanied by a small loss of secondary structure.

PMID: 12044909 [PubMed - indexed for MEDLINE]