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Analytical and computational approaches to define the Aspergillus niger secretome.

Author(s): Tsang A, Butler G, Powlowski J, Panisko EA, Baker SE

Fungal Genet Biol. 2009 Mar;46 Suppl 1:S153-S160 Authors: Tsang A, Butler G, Powlowski J, Panisko EA, Baker SE

Article GUID: 19618504
Transcriptome and exoproteome analysis of utilization of plant-derived biomass by Myceliophthora thermophila.

Author(s): Kolbusz MA, Di Falco M, Ishmael N, Marqueteau S, Moisan MC, Baptista CDS, Powlowski J, Tsang A

Fungal Genet Biol. 2014 Nov;72:10-20 Authors: Kolbusz MA, Di Falco M, Ishmael N, Marqueteau S, Moisan MC, Baptista CDS, Powlowski J, Tsang A

Article GUID: 24881579
Genetics of mating in members of the Chaetomiaceae as revealed by experimental and genomic characterization of reproduction in Myceliophthora heterothallica.

Author(s): Hutchinson MI, Powell AJ, Tsang A, O'Toole N, Berka RM, Barry K, Grigoriev IV, Natvig DO

Fungal Genet Biol. 2016 Jan;86:9-19 Authors: Hutchinson MI, Powell AJ, Tsang A, O'Toole N, Berka RM, Barry K, Grigoriev IV, Natvig DO

Article GUID: 26608618
Title:Analytical and computational approaches to define the Aspergillus niger secretome.
Authors:Tsang A,  Butler G,  Powlowski J,  Panisko EA,  Baker SE
Link:https://www.ncbi.nlm.nih.gov/pubmed/19618504?dopt=Abstract
DOI:10.1016/j.fgb.2008.07.014
Category:Fungal Genet Biol
PMID:19618504
Dept Affiliation: BIOLOGY
1 Centre for Structural and Functional Genomics, Concordia University, 7141 Sherbrooke West, Montréal, Québec, Canada H4B 1R6, Department of Biology, Concordia University, Montréal, Québec, Canada. tsang@gene.concordia.ca

Description:

Analytical and computational approaches to define the Aspergillus niger secretome.

Fungal Genet Biol. 2009 Mar;46 Suppl 1:S153-S160

Authors: Tsang A, Butler G, Powlowski J, Panisko EA, Baker SE

Abstract

We used computational and mass spectrometric approaches to characterize the Aspergillus niger secretome.The 11,200 gene models predicted in the genome of A. niger strain ATCC 1015 were the data source for the analysis. Depending on the computational methods used, 691 to 881 proteins were predicted to be secreted proteins. We cultured A. niger in six different media and analyzed the extracellular proteins produced using mass spectrometry. A total of 222 proteins were identified, with 39 proteins expressed under all six conditions and 74 proteins expressed under only one condition. The secreted proteins identified by mass spectrometry were used to guide the correction of about 20 gene models. Additional analysis focused on extracellular enzymes of interest for biomass processing. Of the 63 glycoside hydrolases predicted to be capable of hydrolyzing cellulose, hemicellulose or pectin, 94% of the exo-acting enzymes and only 18% of the endo-acting enzymes were experimentally detected.

PMID: 19618504 [PubMed - indexed for MEDLINE]