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W361R mutation in GaaR, the regulator of D-galacturonic acid-responsive genes, leads to constitutive production of pectinases in Aspergillus niger.

Author(s): Alazi E, Niu J, Otto SB, Arentshorst M, Pham TTM, Tsang A, Ram AFJ

Microbiologyopen. 2019 May;8(5):e00732 Authors: Alazi E, Niu J, Otto SB, Arentshorst M, Pham TTM, Tsang A, Ram AFJ

Article GUID: 30298571
Title:W361R mutation in GaaR, the regulator of D-galacturonic acid-responsive genes, leads to constitutive production of pectinases in Aspergillus niger.
Authors:Alazi E,  Niu J,  Otto SB,  Arentshorst M,  Pham TTM,  Tsang A,  Ram AFJ
Link:https://www.ncbi.nlm.nih.gov/pubmed/30298571?dopt=Abstract
DOI:10.1002/mbo3.732
Category:Microbiologyopen
PMID:30298571
Dept Affiliation: CSFG
1 Molecular Microbiology and Biotechnology, Institute of Biology Leiden, Leiden University, Leiden, The Netherlands.
2 Centre for Structural and Functional Genomics, Concordia University, Montreal, Québec, Canada.

Description:

W361R mutation in GaaR, the regulator of D-galacturonic acid-responsive genes, leads to constitutive production of pectinases in Aspergillus niger.

Microbiologyopen. 2019 May;8(5):e00732

Authors: Alazi E, Niu J, Otto SB, Arentshorst M, Pham TTM, Tsang A, Ram AFJ

Abstract

Polysaccharides present in plant biomass, such as pectin, are the main carbon source for filamentous fungi. Aspergillus niger naturally secretes pectinases to degrade pectin and utilize the released monomers, mainly D-galacturonic acid. The transcriptional activator GaaR, the repressor of D-galacturonic acid utilization GaaX, and the physiological inducer 2-keto-3-deoxy-L-galactonate play important roles in the transcriptional regulation of D-galacturonic acid-responsive genes, which include the genes encoding pectinases. In this study, we described the mutations found in gaaX and gaaR that enabled constitutive (i.e., inducer-independent) expression of pectinases by A. niger. Using promoter-reporter strains (PpgaX-amdS) and polygalacturonic acid plate assays, we showed that W361R mutation in GaaR results in constitutive production of pectinases. Analysis of subcellular localization of C-terminally eGFP-tagged GaaR/GaaRW 361R revealed important differences in nuclear accumulation of N- versus C-terminally eGFP-tagged GaaR.

PMID: 30298571 [PubMed - in process]