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Importin-binding mediates the intramolecular regulation of anillin during cytokinesis.

Author(s): Beaudet D, Pham N, Skaik N, Piekny A

Mol Biol Cell. 2020 Apr 02;:mbcE20010006 Authors: Beaudet D, Pham N, Skaik N, Piekny A

Article GUID: 32238082
Hof1 plays a checkpoint related role in MMS induced DNA damage response in Candida albicans.

Author(s): Feng J, Islam A, Bean B, Feng J, Sparapani S, Shrivastava M, Goyal A, Omran RP, Mallick J, Whiteway M

Mol Biol Cell. 2020 Jan 15;:mbcE19060316 Authors: Feng J, Islam A, Bean B, Feng J, Sparapani S, Shrivastava M, Goyal A, Omran RP, Mallick J, Whiteway M

Article GUID: 31940254
Characterization of a novel separase-interacting protein and candidate new securin, Eip1p, in the fungal pathogen Candida albicans

Author(s): Sparapani S; Bachewich C;

Proper chromosome segregation is crucial for maintaining genomic stability and dependent on separase, a conserved and essential cohesin protease. Securins are key regulators of separases, but remain elusive in many organisms due to sequence divergence. Here...

Article GUID: 31411946
Active Ran regulates anillin function during cytokinesis.

Author(s): Beaudet D, Akhshi T, Phillipp J, Law C, Piekny A

Mol Biol Cell. 2017 Nov 15;28(24):3517-3531 Authors: Beaudet D, Akhshi T, Phillipp J, Law C, Piekny A

Article GUID: 28931593
The Na+(K+)/H+ exchanger Nhx1 controls multivesicular body-vacuolar lysosome fusion.

Author(s): Karim MA, Brett CL

Mol Biol Cell. 2018 02 01;29(3):317-325 Authors: Karim MA, Brett CL

Article GUID: 29212874
The adaptor protein Ste50 directly modulates yeast MAPK signaling specificity through differential connections of its RA domain.

Author(s): Sharmeen N, Sulea T, Whiteway M, Wu C

Mol Biol Cell. 2019 03 15;30(6):794-807 Authors: Sharmeen N, Sulea T, Whiteway M, Wu C

Article GUID: 30650049
Title:Importin-binding mediates the intramolecular regulation of anillin during cytokinesis.
Authors:Beaudet D,  Pham N,  Skaik N,  Piekny A
Link:https://www.ncbi.nlm.nih.gov/pubmed/32238082?dopt=Abstract
DOI:10.1091/mbc.E20-01-0006
Category:Mol Biol Cell
PMID:32238082
Dept Affiliation: BIOLOGY
1 Department of Bioengineering, McGill University, Montreal, QC, Canada, H3A 0G4.
2 Department of Biology, Concordia University, Montreal, QC, Canada, H4B 1R6.

Description:

Importin-binding mediates the intramolecular regulation of anillin during cytokinesis.

Mol Biol Cell. 2020 Apr 02;:mbcE20010006

Authors: Beaudet D, Pham N, Skaik N, Piekny A

Abstract

Cytokinesis occurs by the ingression of an actomyosin ring that cleaves a cell into two daughters. This process is tightly controlled to avoid aneuploidy, and we previously showed that active Ran coordinates ring positioning with chromatin. Active Ran is high around chromatin, and forms an inverse gradient to cargo-bound importins. We found that the ring component anillin contains a nuclear localization signal (NLS) that binds to importin and is required for its function during cytokinesis. Here we reveal the mechanism whereby importin-binding favors a conformation required for anillin's recruitment to the equatorial cortex. Active RhoA binds to the RhoA-binding domain (RBD) causing an increase in accessibility of the nearby C2 domain containing the NLS. Importin-binding subsequently stabilizes a conformation that favors interactions for cortical recruitment. In addition to revealing a novel mechanism for the importin-mediated regulation of a cortical protein, we also show how importin-binding positively regulates protein function.

PMID: 32238082 [PubMed - as supplied by publisher]