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Characterization of Phase I and Glucuronide Phase II Metabolites of 17 Mycotoxins Using Liquid Chromatography-High-Resolution Mass Spectrometry

Author(s): Slobodchikova I; Sivakumar R; Rahman MS; Vuckovic D;

Routine mycotoxin biomonitoring methods do not include many mycotoxin phase I and phase II metabolites, which may significantly underestimate mycotoxin exposure especially for heavily metabolized mycotoxins. Additional research efforts are also needed to me...

Article GUID: 31344861
Evaluation of D10-Leu metabolic labeling coupled with MALDI-MS analysis in studying the response of the yeast proteome to H2O2 challenge

Author(s): Jiang H; English AM;

An efficient D10-Leu metabolic-labeling method combined with isotope-ratio quantitation by MALDI-TOF MS was used to probe the response of the yeast proteome to H2O2. Control cultures correct for effects not associated with H2O2 challenge. A stress-response ...

Article GUID: 17022625
Reduction and S-nitrosation of the neuropeptide oxytocin: implications for its biological function

Author(s): Roy JF; Chrétien MN; Woodside B; English AM;

Oxytocin (OT; Cys-Tyr-Ile-Gln-Asn-Cys-Pro-leu-Gly), a posterior pituitary peptide hormone, is characterized by a Cys1-Cys6 disulfide bond in its stable, isolated state. This paper describes a simple, one-step method for the production of OT in its reduced, ...

Article GUID: 17692543
Human blood cell levels of 5-hydroxymethylcytosine (5hmC) decline with age, partly related to acquired mutations in TET2

Author(s): Buscarlet M; Tessier A; Provost S; Mollica L; Busque L;

Epigenetic alteration may play a role in age-associated dysfunction of stem cells and predispose to the development of hematological cancers. We analyzed global levels of hematopoietic 5-hydroxymethylcytosine (5hmC) and 5-methylcytosine (5mC) in a cross-sec...

Article GUID: 27475703
Title:Characterization of Phase I and Glucuronide Phase II Metabolites of 17 Mycotoxins Using Liquid Chromatography-High-Resolution Mass Spectrometry
Authors:Slobodchikova I,  Sivakumar R,  Rahman MS,  Vuckovic D, 
Link:https://pubmed.ncbi.nlm.nih.gov/31344861/
DOI:10.3390/toxins11080433
Category:Toxins (Basel)
PMID:31344861
Dept Affiliation: CBAMS
1 Department of Chemistry and Biochemistry, Concordia University, 7141 Sherbrooke Street West, Montreal, QC H4B 1R6, Canada.
2 Centre for Biological Applications of Mass Spectrometry, Concordia University, 7141 Sherbrooke Street West, Montreal, QC H4B 1R6, Canada.
3 Department of Chemistry and Biochemistry, Concordia University, 7141 Sherbrooke Street West, Montreal, QC H4B 1R6, Canada. dajana.vuckovic@concordia.ca.
4 Centre for Biological Applications of Mass Spectrometry, Concordia University, 7141 Sherbrooke Street West, Montreal, QC H4B 1R6, Canada. dajana.vuckovic@concordia.ca.

Description:

Routine mycotoxin biomonitoring methods do not include many mycotoxin phase I and phase II metabolites, which may significantly underestimate mycotoxin exposure especially for heavily metabolized mycotoxins. Additional research efforts are also needed to measure metabolites in vivo after exposure and to establish which mycotoxin metabolites should be prioritized for the inclusion during large-scale biomonitoring efforts. The objective of this study was to perform human in vitro microsomal incubations of 17 mycotoxins and systematically characterize all resulting metabolites using liquid chromatography-high-resolution mass spectrometry (LC-HRMS). The results obtained were then used to build a comprehensive LC-MS library and expand a validated 17-mycotoxin method for exposure monitoring to screening of additional 188 metabolites, including 100 metabolites reported for the first time. The final method represents one of the most comprehensive LC-HRMS methods for mycotoxin biomonitoring or metabolism/fate studies.