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Concordia Publications:

Title Authors PubMed ID
1 Characterization of Phase I and Glucuronide Phase II Metabolites of 17 Mycotoxins Using Liquid Chromatography-High-Resolution Mass Spectrometry Slobodchikova I; Sivakumar R; Rahman MS; Vuckovic D; 31344861
CBAMS
2 Evaluation of D10-Leu metabolic labeling coupled with MALDI-MS analysis in studying the response of the yeast proteome to H2O2 challenge Jiang H; English AM; 17022625
CBAMS
3 Reduction and S-nitrosation of the neuropeptide oxytocin: implications for its biological function Roy JF; Chrétien MN; Woodside B; English AM; 17692543
CBAMS
4 Human blood cell levels of 5-hydroxymethylcytosine (5hmC) decline with age, partly related to acquired mutations in TET2 Buscarlet M; Tessier A; Provost S; Mollica L; Busque L; 27475703
CBAMS

 

Title:Evaluation of D10-Leu metabolic labeling coupled with MALDI-MS analysis in studying the response of the yeast proteome to H2O2 challenge
Authors:Jiang HEnglish AM
Link:https://pubmed.ncbi.nlm.nih.gov/17022625/
DOI:10.1021/pr060019m
Publication:Journal of proteome research
Keywords:
PMID:17022625 Category:J Proteome Res Date Added:2019-06-19
Dept Affiliation: CBAMS
1 Centre for Biological Applications of Mass Spectrometry, Department of Chemistry and Biochemistry, Concordia University, Montreal, Quebec, Canada H4B 1R6.

Description:

An efficient D10-Leu metabolic-labeling method combined with isotope-ratio quantitation by MALDI-TOF MS was used to probe the response of the yeast proteome to H2O2. Control cultures correct for effects not associated with H2O2 challenge. A stress-response index to H2O2 (SRIH2O2) is defined, and values are reported for seven proteins at 45-225 min following exposure to 0.4 mM H2O2. The time course of protein accumulation in unstressed cells following the H10- to D10-SCD switch suggests that proteome responses at <45 min could be monitored by addition of excess D10-Leu to H10-cultures.





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