Keyword search (4,163 papers available)

"Adipose tissue" Keyword-tagged Publications:

Title Authors PubMed ID
1 The age of obesity onset affects changes in subcutaneous adipose tissue macrophages and T cells after weight loss Murphy J; Morais JA; Tsoukas MA; Cooke AB; Daskalopoulou SS; Santosa S; 40831565
SOH
2 Is Adipose Tissue Inflammation the Culprit of Obesity-Associated Comorbidities? Turner L; Wanasinghe AI; Brunori P; Santosa S; 40533358
SOH
3 Regional primary preadipocyte characteristics in humans with obesity and type 2 diabetes mellitus Plissonneau C; Santosa S; 39553621
SOH
4 Sex differences in regional adipose tissue depots pose different threats for the development of Type 2 diabetes in males and females Kerri Z Delaney 34985183
PERFORM
5 Altered immunometabolism in adipose tissue: a major contributor to the ageing process? Delaney KZ; Gillespie ZE; Murphy J; Wang C; 34159597
PERFORM
6 Putting ATM to BED: How Adipose Tissue Macrophages Are Affected by Bariatric Surgery, Exercise, and Dietary Fatty Acids Turner L; Santosa S; 33979430
PERFORM
7 Association between rs174537 FADS1 polymorphism and immune cell profiles in abdominal and femoral subcutaneous adipose tissue: an exploratory study in adults with obesity Wang C; Murphy J; Delaney KZ; Khor N; Morais JA; Tsoukas MA; Lowry DE; Mutch DM; Santosa S; 33595419
PERFORM
8 A reliable, reproducible flow cytometry protocol for immune cell quantification in human adipose tissue. Delaney KZ, Dam V, Murphy J, Morais JA, Denis R, Atlas H, Pescarus R, Garneau PY, Santosa S 32926866
PERFORM
9 Acetyl-CoA regulation, OXPHOS integrity and leptin level are different in females with different onsets of obesity. Tam BT, Murphy J, Khor N, Morais JA, Santosa S 32808657
PERFORM
10 From neutrophils to macrophages: differences in regional adipose tissue depots. Dam V, Sikder T, Santosa S 26667065
PERFORM
11 Factors associated with adipocyte size reduction after weight loss interventions for overweight and obesity: a systematic review and meta-regression. Murphy J, Moullec G, Santosa S 28081776
PERFORM

 

Title:A reliable, reproducible flow cytometry protocol for immune cell quantification in human adipose tissue.
Authors:Delaney KZ,  Dam V,  Murphy J,  Morais JA,  Denis R,  Atlas H,  Pescarus R,  Garneau PY,  Santosa S
Link:https://www.ncbi.nlm.nih.gov/pubmed/32926866
DOI:10.1016/j.ab.2020.113951
Publication:Analytical biochemistry
Keywords:Adipose tissue;  Flow cytometry;  Humans;  Immune cells;  Inflammation;  Obesity; 
PMID:32926866 Category:Anal Biochem Date Added:2020-09-15
Dept Affiliation: PERFORM
1 Department of Health, Kinesiology, and Applied Physiology, Concordia University, 7141 Sherbrooke St W, Montreal, QC, H4B 1R6, Canada; Metabolism, Obesity, Nutrition Lab, PERFORM Centre, Concordia University, 7200 Sherbrooke St W, Montreal, QC, H4B 1R6, Canada; Centre de Recherche - Axe Maladies Chroniques, Centre Intégré Universitaire de Santé et de Services Sociaux Du Nord-de-l'Ile-de-Montreal, Hôpital Du Sacré-Coeur de Montreal, 5400 Boul Gouin O, Montréal, QC, H4J 1C5, Canada.
2 Department of Health, Kinesiology, and Applied Physiology, Concordia University, 7141 Sherbrooke St W, Montreal, QC, H4B 1R6, Canada; Division of Geriatric Medicine, McGill University Health Centre - Royal Victoria Hospital, 1001, Décarie, Montreal, Québec, H4A 3J1, Canada.
3 Departement Du Chirurgie, Hôpital Du Sacré-Coeur de Montréal, 5400 Boul Gouin O, Montréal, QC, H4J 1C5, Canada.
4 Department of Health, Kinesiology, and Applied Physiology, Concordia University, 7141 Sherbrooke St W, Montreal, QC, H4B 1R6, Canada; Metabolism, Obesity, Nutrition Lab, PERFORM Centre, Concordia University, 7200 Sherbrooke St W, Montreal, QC, H4B 1R6, Canada; Centre de Recherche - Axe Maladies Chroniques, Centre Intégré Universitaire de Santé et de Services Sociaux Du Nord-de-l'Ile-de-Montreal, Hôpital Du Sacré-Coeur de Montreal, 5400 Boul Gouin O, Montréal, QC, H4J 1C5, Canada. Electronic address: s.santosa@concordia.ca.

Description:

A reliable, reproducible flow cytometry protocol for immune cell quantification in human adipose tissue.

Anal Biochem. 2020 Sep 11; :113951

Authors: Delaney KZ, Dam V, Murphy J, Morais JA, Denis R, Atlas H, Pescarus R, Garneau PY, Santosa S

Abstract

The ability to accurately identify and quantify immune cell populations within adipose tissue is important in understanding the role of immune cells in metabolic disease risk. Flow cytometry is the gold standard method for immune cell quantification. However, quantification of immune cells from adipose tissue presents a number of challenges because of the complexities of working with an oily substance and the rapid deterioration of immune cell viability before analysis can be performed. Here we present a highly reproducible flow cytometry protocol for the quantification of immune cells in human adipose tissue, which overcomes these issues.

PMID: 32926866 [PubMed - as supplied by publisher]




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