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Multi-tissue patterning drives anterior morphogenesis of the C. elegans embryo.

Author(s): Grimbert S, Mastronardi K, Richard V, Christensen R, Law C, Zardoui K, Fay D, Piekny A

Complex structures derived from multiple tissue types are challenging to study in vivo, and our knowledge of how cells from different tissues are coordinated is limited. Model organisms have proven invaluable for improving our understanding of how chemical ...

Article GUID: 33309948
The phenotype associated with variants in TANGO2 may be explained by a dual role of the protein in ER-to-Golgi transport and at the mitochondria.

Author(s): Milev MP, Saint-Dic D, Zardoui K, Klopstock T, Law C, Distelmaier F, Sacher M

TANGO2 variants result in a complex disease phenotype consisting of recurrent crisis-induced rhabdomyolysis, encephalopathy, seizures, lactic acidosis, hypoglycemia, and cardiac arrhythmias. Although first described in a fruit fly model as a protein necessa...

Article GUID: 32909282
Photosystem Biogenesis Is Localized to the Translation Zone in the Chloroplast of Chlamydomonas.

Author(s): Sun Y, Valente-Paterno MI, Bakhtiari S, Law C, Zhan Y, Zerges W

Photosystem Biogenesis Is Localized to the Translation Zone in the Chloroplast of Chlamydomonas.

Plant Cell. 2019 Oct 07;:

Authors: Sun Y, Valente-Paterno MI, Bakhtiari S, Law C, Zhan Y, Zerges W

Abstract
Intracellular pro...

Article GUID: 31591163
Active Ran regulates anillin function during cytokinesis.

Author(s): Beaudet D, Akhshi T, Phillipp J, Law C, Piekny A

Mol Biol Cell. 2017 Nov 15;28(24):3517-3531 Authors: Beaudet D, Akhshi T, Phillipp J, Law C, Piekny A

Article GUID: 28931593
Title:Active Ran regulates anillin function during cytokinesis.
Authors:Beaudet D,  Akhshi T,  Phillipp J,  Law C,  Piekny A
Link:https://www.ncbi.nlm.nih.gov/pubmed/28931593?dopt=Abstract
DOI:10.1091/mbc.E17-04-0253
Category:Mol Biol Cell
PMID:28931593
Dept Affiliation: BIOLOGY
1 Department of Biology, Concordia University, Montreal, QC H4B 1R6, Canada.
2 Program in Cell Biology, the Hospital for Sick Children, Toronto, ON M5G 0A4, Canada.
3 Department of Biochemistry, University of Toronto, Toronto, ON M5S 1A8, Canada.
4 Centre for Microscopy and Cellular Imaging, Concordia University, Montreal, QC H4B 1R6, Canada.
5 Program in Cell Biology, the Hospital for Sick Children, Toronto, ON M5G 0A4, Canada alisa.piekny@concordia.ca.

Description:

Active Ran regulates anillin function during cytokinesis.

Mol Biol Cell. 2017 Nov 15;28(24):3517-3531

Authors: Beaudet D, Akhshi T, Phillipp J, Law C, Piekny A

Abstract

Cytokinesis cleaves a cell into two daughters at the end of mitosis, and must be spatially coordinated with chromosome segregation to prevent aneuploidy. The dogma is that the mitotic spindle governs the assembly and constriction of an actomyosin ring. Here, we reveal a function for active Ran in spatially restricting the ring. Our model is that during anaphase, "free" importins, whose gradient inversely correlates with active Ran and chromatin position, function as a molecular ruler for the recruitment and localization of anillin, a contractile protein and a crucial regulator of cytokinesis. We found that decreasing Ran-GTP levels or tethering active Ran to the equatorial membrane affects anillin's localization and causes cytokinesis phenotypes. Anillin contains a conserved nuclear localization signal (NLS) at its C-terminus that binds to importin-ß and is required for cortical polarity and cytokinesis. Mutating the NLS decreases anillin's cortical affinity, causing it to be more dominantly regulated by microtubules. Anillin contains a RhoA-GTP binding domain, which autoinhibits the NLS and the neighboring microtubule-binding domain, and RhoA-GTP binding may relieve this inhibition during mitosis. Retention of the C-terminal NLS in anillin homologues suggests that this is a conserved mechanism for controlling anillin function.

PMID: 28931593 [PubMed - indexed for MEDLINE]