Keyword search (3,448 papers available)


The effect of step-feeding distribution ratio on high concentration perchlorate removal performance in ABR system with heterotrophic combined sulfur autotrophic process.

Author(s): Li H, Li K, Guo J, Chen Z, Han Y, Song Y, Lu C, Hou Y, Zhang D, Zhang Y

In a lab-scale anaerobic baffled reactor (ABR) with eight compartments, the heterotrophic and sulfur autotrophic processes were combined to remove perchlorate. And then, the step-feeding distribution ratio of the heterotrophic perchlorate reduction unit (HP...

Article GUID: 33485237
Evaluation of System Modelling Techniques for Waste Identification in Lean Healthcare Applications.

Author(s): Alkaabi M, Simsekler MCE, Jayaraman R, Al Kaf A, Ghalib H, Quraini D, Ellahham S, Tuzcu EM, Demirli K...

Purpose: Waste identification plays a vital role in lean healthcare applications. While the value stream map (VSM) is among the most commonly used tools for waste identification, it may be limited ...

Article GUID: 33447104
One Cell, One Drop, One Click: Hybrid Microfluidics for Mammalian Single Cell Isolation.

Author(s): Samlali K, Ahmadi F, Quach ABV, Soffer G, Shih SCC

Small. 2020 Jul 23;:e2002400 Authors: Samlali K, Ahmadi F, Quach ABV, Soffer G, Shih SCC

Article GUID: 32705796
Reflective and Reflexive Stress Responses of Older Adults to Three Gaming Experiences In Relation to Their Cognitive Abilities: Mixed Methods Crossover Study.

Author(s): Khalili-Mahani N, Assadi A, Li K, Mirgholami M, Rivard ME, Benali H, Sawchuk K, De Schutter B

JMIR Ment Health. 2020 Mar 26;7(3):e12388 Authors: Khalili-Mahani N, Assadi A, Li K, Mirgholami M, Rivard ME, Benali H, Sawchuk K, De Schutter B

Article GUID: 32213474
Dual Reduction/Acid-Responsive Disassembly and Thermoresponsive Tunability of Degradable Double Hydrophilic Block Copolymer.

Author(s): Maruya-Li K, Shetty C, Moini Jazani A, Arezi N, Oh JK

ACS Omega. 2020 Feb 25;5(7):3734-3742 Authors: Maruya-Li K, Shetty C, Moini Jazani A, Arezi N, Oh JK

Article GUID: 32118189
Facile Strategies to Synthesize Dual Location Dual Acidic pH/Reduction-Responsive Degradable Block Copolymers Bearing Acetal/Disulfide Block Junctions and Disulfide Pendants.

Author(s): Jazani AM, Arezi N, Maruya-Li K, Jung S, Oh JK

ACS Omega. 2018 Aug 31;3(8):8980-8991 Authors: Jazani AM, Arezi N, Maruya-Li K, Jung S, Oh JK

Article GUID: 31459031
Testing continuity and activity variables as predictors of positive and negative affect in retirement.

Author(s): Pushkar D, Chaikelson J, Conway M, Etezadi J, Giannopoulus C, Li K, Wrosch C

J Gerontol B Psychol Sci Soc Sci. 2010 Jan;65B(1):42-9 Authors: Pushkar D, Chaikelson J, Conway M, Etezadi J, Giannopoulus C, Li K, Wrosch C

Article GUID: 19875749
A Crowdsensing Based Analytical Framework for Perceptional Degradation of OTT Web Browsing.

Author(s): Li K, Wang H, Xu X, Du Y, Liu Y, Ahmad MO

Sensors (Basel). 2018 May 15;18(5): Authors: Li K, Wang H, Xu X, Du Y, Liu Y, Ahmad MO

Article GUID: 29762493
Guidelines for Gait Assessments in the Canadian Consortium on Neurodegeneration in Aging (CCNA).

Author(s): Cullen S, Montero-Odasso M, Bherer L, Almeida Q, Fraser S, Muir-Hunter S, Li K, Liu-Ambrose T, McGibbon CA, McIlroy W, Middleton LE, Sarquis...

Can Geriatr J. 2018 Jun;21(2):157-165 Authors: Cullen S, Montero-Odasso M, Bherer L, Almeida Q, Fraser S, Muir-Hunter S, Li K, Liu-Ambrose T, McGibbon CA, McIlroy W, Middleton LE, Sarquis-Adamson ...

Article GUID: 29977431
An integrated droplet-digital microfluidic system for on-demand droplet creation, mixing, incubation, and sorting.

Author(s): Ahmadi F, Samlali K, Vo PQN, Shih SCC

Lab Chip. 2019 01 29;19(3):524-535 Authors: Ahmadi F, Samlali K, Vo PQN, Shih SCC

Article GUID: 30633267
A combined heterotrophic and sulfur-based autotrophic process to reduce high concentration perchlorate via anaerobic baffled reactors: Performance advantages of a step-feeding strategy.

Author(s): Li K, Guo J, Li H, Han Y, Chen Z, Song Y, Xing Y, Zhang C

Bioresour Technol. 2019 May;279:297-306 Authors: Li K, Guo J, Li H, Han Y, Chen Z, Song Y, Xing Y, Zhang C

Article GUID: 30738356
Effects of pool size and spacing on burning rate and flame height of two square heptane pool fires.

Author(s): Wan H, Gao Z, Ji J, Zhang Y, Li K, Wang L

J Hazard Mater. 2019 May 05;369:116-124 Authors: Wan H, Gao Z, Ji J, Zhang Y, Li K, Wang L

Article GUID: 30776594
Big Data-Driven Cellular Information Detection and Coverage Identification.

Author(s): Wang H, Xie S, Li K, Ahmad MO

Sensors (Basel). 2019 Feb 22;19(4): Authors: Wang H, Xie S, Li K, Ahmad MO

Article GUID: 30813353
SYNERGIC TRIAL (SYNchronizing Exercises, Remedies in Gait and Cognition) a multi-Centre randomized controlled double blind trial to improve gait and cognition in mild cognitive impairment.

Author(s): Montero-Odasso M, Almeida QJ, Burhan AM, Camicioli R, Doyon J, Fraser S, Li K, Liu-Ambrose T, Middleton L, Muir-Hunter S, McIlroy W, Morais ...

BMC Geriatr. 2018 04 16;18(1):93 Authors: Montero-Odasso M, Almeida QJ, Burhan AM, Camicioli R, Doyon J, Fraser S, Li K, Liu-Ambrose T, Middleton L, Muir-Hunter S, McIlroy W, Morais JA, Pieruccini...

Article GUID: 29661156
A cross-sectional analysis on the effects of age on dual tasking in typically developing children.

Author(s): Saxena S, Majnemer A, Li K, Beauchamp M, Gagnon I

Psychol Res. 2019 Feb;83(1):104-115 Authors: Saxena S, Majnemer A, Li K, Beauchamp M, Gagnon I

Article GUID: 30506524
Brain gray matter volume associations with gait speed and related structural covariance networks in cognitively healthy individuals and in patients with mild cognitive impairment: A cross-sectional study.

Author(s): Beauchet O, Montembeault M, Barden JM, Szturm T, Bherer L, Liu-Ambrose T, Chester VL, Li K, Helbostad JL, Allali G, Canadian Gait Consortium

Exp Gerontol. 2019 Jul 15;122:116-122 Authors: Beauchet O, Montembeault M, Barden JM, Szturm T, Bherer L, Liu-Ambrose T, Chester VL, Li K, Helbostad JL, Allali G, Canadian Gait Consortium

Article GUID: 31075383
Title:One Cell, One Drop, One Click: Hybrid Microfluidics for Mammalian Single Cell Isolation.
Authors:Samlali K,  Ahmadi F,  Quach ABV,  Soffer G,  Shih SCC
Link:https://www.ncbi.nlm.nih.gov/pubmed/32705796
DOI:10.1002/smll.202002400
Category:Small
PMID:32705796
Dept Affiliation: BIOLOGY
1 Department of Electrical and Computer Engineering, Concordia University, Montréal, Québec, H3G 1M8, Canada.
2 Centre for Applied Synthetic Biology, Concordia University, Montréal, Québec, H4B 1R6, Canada.
3 Department of Biology, Concordia University, Montréal, Québec, H4B 1R6, Canada.

Description:

One Cell, One Drop, One Click: Hybrid Microfluidics for Mammalian Single Cell Isolation.

Small. 2020 Jul 23;:e2002400

Authors: Samlali K, Ahmadi F, Quach ABV, Soffer G, Shih SCC

Abstract

Generating a stable knockout cell line is a complex process that can take several months to complete. In this work, a microfluidic method that is capable of isolating single cells in droplets, selecting successful edited clones, and expansion of these isoclones is introduced. Using a hybrid microfluidics method, droplets in channels can be individually addressed using a co-planar electrode system. In the hybrid microfluidics device, it is shown that single cells can be trapped and subsequently encapsulate them on demand into pL-sized droplets. Furthermore, droplets containing single cells are either released, kept in the traps, or merged with other droplets by the application of an electric potential to the electrodes that is actuated through an in-house user interface. This high precision control is used to successfully sort and recover single isoclones to establish monoclonal cell lines, which is demonstrated with a heterozygous NCI-H1299 lung squamous cell population resulting from loss-of-function eGFP and RAF1 gene knockout transfections.

PMID: 32705796 [PubMed - as supplied by publisher]