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Hof1 plays a checkpoint related role in MMS induced DNA damage response in Candida albicans.

Author(s): Feng J, Islam A, Bean B, Feng J, Sparapani S, Shrivastava M, Goyal A, Omran RP, Mallick J, Whiteway M

Mol Biol Cell. 2020 Jan 15;:mbcE19060316 Authors: Feng J, Islam A, Bean B, Feng J, Sparapani S, Shrivastava M, Goyal A, Omran RP, Mallick J, Whiteway M

Article GUID: 31940254
RNA sequencing reveals an additional Crz1-binding motif in promoters of its target genes in the human fungal pathogen Candida albicans.

Author(s): Xu H, Fang T, Omran RP, Whiteway M, Jiang L

Cell Commun Signal. 2020 Jan 03;18(1):1 Authors: Xu H, Fang T, Omran RP, Whiteway M, Jiang L

Article GUID: 31900175
Screening of Candida albicans GRACE library revealed a unique pattern of biofilm formation under repression of the essential gene ILS1.

Author(s): Costa ACBP, Omran RP, Correia-Mesquita TO, Dumeaux V, Whiteway M

Sci Rep. 2019 Jun 24;9(1):9187 Authors: Costa ACBP, Omran RP, Correia-Mesquita TO, Dumeaux V, Whiteway M

Article GUID: 31235750
MAP Kinase Regulation of the Candida albicans Pheromone Pathway.

Author(s): Rastghalam G, Omran RP, Alizadeh M, Fulton D, Mallick J, Whiteway M

mSphere. 2019 02 20;4(1): Authors: Rastghalam G, Omran RP, Alizadeh M, Fulton D, Mallick J, Whiteway M

Article GUID: 30787119
Mms21: A Putative SUMO E3 Ligase in Candida albicans That Negatively Regulates Invasiveness and Filamentation, and Is Required for the Genotoxic and Cellular Stress Response.

Author(s): Islam A, Tebbji F, Mallick J, Regan H, Dumeaux V, Omran RP, Whiteway M

Genetics. 2019 02;211(2):579-595 Authors: Islam A, Tebbji F, Mallick J, Regan H, Dumeaux V, Omran RP, Whiteway M

Article GUID: 30530734
Put3 Positively Regulates Proline Utilization in Candida albicans.

Author(s): Tebung WA, Omran RP, Fulton DL, Morschhäuser J, Whiteway M

mSphere. 2017 Nov-Dec;2(6): Authors: Tebung WA, Omran RP, Fulton DL, Morschhäuser J, Whiteway M

Article GUID: 29242833
Title:Mms21: A Putative SUMO E3 Ligase in Candida albicans That Negatively Regulates Invasiveness and Filamentation, and Is Required for the Genotoxic and Cellular Stress Response.
Authors:Islam A,  Tebbji F,  Mallick J,  Regan H,  Dumeaux V,  Omran RP,  Whiteway M
Link:https://www.ncbi.nlm.nih.gov/pubmed/30530734?dopt=Abstract
DOI:10.1534/genetics.118.301769
Category:Genetics
PMID:30530734
Dept Affiliation: PERFORM
1 Department of Biology, Concordia University, Montreal, Quebec H4B 1R6, Canada.
2 Infectious Centre de Recherche en Infectiologie (CRI), Centre Hospitalier Universitaire de Quebec (CHUQ) Research Center, University Laval, Quebec City, Quebec G1V 4G2, Canada.
3 Prevention Evaluation Rehabilitation Formation (PERFORM) Centre, Concordia University, Montreal, Quebec H4B 1R6, Canada.
4 Department of Biology, Concordia University, Montreal, Quebec H4B 1R6, Canada malcolm.whiteway@concordia.ca.

Description:

Mms21: A Putative SUMO E3 Ligase in Candida albicans That Negatively Regulates Invasiveness and Filamentation, and Is Required for the Genotoxic and Cellular Stress Response.

Genetics. 2019 02;211(2):579-595

Authors: Islam A, Tebbji F, Mallick J, Regan H, Dumeaux V, Omran RP, Whiteway M

Abstract

In the life cycle of the fungal pathogen Candida albicans, the formation of filamentous cells is a differentiation process that is critically involved in host tissue invasion, and in adaptation to host cell and environmental stresses. Here, we have used the Gene Replacement And Conditional Expression library to identify genes controlling invasiveness and filamentation; conditional repression of the library revealed 69 mutants that triggered these processes. Intriguingly, the genes encoding the small ubiquitin-like modifier (SUMO) E3 ligase Mms21, and all other tested members of the sumoylation pathway, were both nonessential and capable of triggering filamentation upon repression, suggesting an important role for sumoylation in controlling filamentation in C. albicans We have investigated Mms21 in detail. Both Mms21 nulls (mms21?/?) and SP [Siz/Pias (protein inhibitor of activated signal transducer and activator of transcription)] domain (SUMO E3 ligase domain)-deleted mutants displayed invasiveness, filamentation, and abnormal nuclear segregation; filament formation occurred even in the absence of the hyphal transcription factor Efg1. Transcriptional analysis of mms21?/? showed an increase in expression from two- to eightfold above that of the wild-type for hyphal-specific genes, including ECE1, PGA13, PGA26, HWP1, ALS1, ALS3, SOD4, SOD5, UME6, and HGC1 The Mms21-deleted mutants were unable to recover from DNA-damaging agents like methyl methane sulfonate, hydroxyurea, hydrogen peroxide, and UV radiation, suggesting that the protein is important for genotoxic stress responses. In addition, the mms21?/? mutant displayed sensitivity to cell wall and thermal stresses, and to different antifungal drugs. All these findings suggest that Mms21 plays important roles in cellular differentiation, DNA damage and cellular stress responses, and in response to antifungal drugs.

PMID: 30530734 [PubMed - indexed for MEDLINE]