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A novel homozygous variant in TRAPPC2L results in a neurodevelopmental disorder and disrupts TRAPP complex function.

Author(s): Al-Deri N, Okur V, Ahimaz P, Milev M, Valivullah Z, Hagen J, Sheng Y, Chung W, Sacher M, Ganapathi M...

BACKGROUND: Next-generation sequencing has facilitated the diagnosis of neurodevelopmental disorders with variable and non-specific clinical findings. Recently, a homozygous missense p.(Asp37Tyr) v...

Article GUID: 32843486
The contribution of dry indoor built environment on the spread of Coronavirus: Data from various Indian states.

Author(s): V AAR, R V, Haghighat F

Coronavirus spread is more serious in urban metropolitan cities compared to rural areas. It is observed from the data on the infection rate available in the various sources that the cold and dry conditions accelerate the spread of coronavirus. In the presen...

Article GUID: 32834934
Ageism and COVID-19: What does our society's response say about us?

Author(s): Fraser S, Lagacé M, Bongué B, Ndeye N, Guyot J, Bechard L, Garcia L, Taler V, CCNA Social Inclusion and Stigma Working Group, Adam S, Beauli...

Age Ageing. 2020 May 06;: Authors: Fraser S, Lagacé M, Bongué B, Ndeye N, Guyot J, Bechard L, Garcia L, Taler V, CCNA Social Inclusion and Stigma Working Group, Adam S, Beaulieu M, Berge...

Article GUID: 32377666
Functional Characterization of Clinical Isolates of the Opportunistic Fungal Pathogen Aspergillus nidulans.

Author(s): Bastos RW, Valero C, Silva LP, Schoen T, Drott M, Brauer V, Silva-Rocha R, Lind A, Steenwyk JL, Rokas A, Rodrigues F, Resendiz-Sharpe A, Lag...

mSphere. 2020 Apr 08;5(2): Authors: Bastos RW, Valero C, Silva LP, Schoen T, Drott M, Brauer V, Silva-Rocha R, Lind A, Steenwyk JL, Rokas A, Rodrigues F, Resendiz-Sharpe A, Lagrou K, Marcet-Houben...

Article GUID: 32269156
Enzymatic assay for GHB determination in forensic matrices.

Author(s): Grenier V, Huppé G, Lamarche M, Mireault P

J Anal Toxicol. 2012 Sep;36(7):523-8 Authors: Grenier V, Huppé G, Lamarche M, Mireault P

Article GUID: 22722059
Characterization and Efficient Management of Big Data in IoT-Driven Smart City Development.

Author(s): Alsaig A, Alagar V, Chammaa Z, Shiri N

Sensors (Basel). 2019 May 28;19(11): Authors: Alsaig A, Alagar V, Chammaa Z, Shiri N

Article GUID: 31141899
Laser-induced micropore formation and modification of cartilage structure in osteoarthritis healing.

Author(s): Sobol E, Baum O, Shekhter A, Wachsmann-Hogiu S, Shnirelman A, Alexandrovskaya Y, Sadovskyy I, Vinokur V

J Biomed Opt. 2017 09 01;22(9):91515 Authors: Sobol E, Baum O, Shekhter A, Wachsmann-Hogiu S, Shnirelman A, Alexandrovskaya Y, Sadovskyy I, Vinokur V

Article GUID: 28564689
Title:Enzymatic assay for GHB determination in forensic matrices.
Authors:Grenier V,  Huppé G,  Lamarche M,  Mireault P
Link:https://www.ncbi.nlm.nih.gov/pubmed/22722059?dopt=Abstract
DOI:10.1093/jat/bks053
Category:J Anal Toxicol
PMID:22722059
Dept Affiliation: MASSSPEC
1 Concordia University, Montreal, Quebec, Canada.

Description:

Enzymatic assay for GHB determination in forensic matrices.

J Anal Toxicol. 2012 Sep;36(7):523-8

Authors: Grenier V, Huppé G, Lamarche M, Mireault P

Abstract

Current procedures for the determination of gamma-hydroxybutyric acid (GHB) require time-consuming extraction and derivatization steps before chromatographic detection, making a high-throughput alternative desirable. Bühlmann Laboratories offers an enzymatic assay for the quantitative determination of GHB in urine and serum. We report the adaptation of this photometric assay to the Thermo Scientific MGC-240 analyzer and its use in the determination of GHB in forensic matrices including urine, whole blood and vitreous humour. Most matrices require only a brief centrifugation before analysis, while blood requires an additional protein precipitation step. A variety of cases (sexual assaults, impaired drivers and death investigations) have been analyzed alongside the gas chromatography-mass spectrometry (GC-MS) reference method. Correlation with the GC-MS has been found to be acceptable, with no false negatives and few false positives, although postmortem samples appear more prone to testing false positive than do antemortem samples. Simple sample preparation and high throughput allow for a significant reduction in analysis time relative to chromatographic methods. This assay is used as a screening method in our laboratory, with a quantitative GC-MS method serving for the confirmation of positive results. To our knowledge, this represents the first evaluation of an enzymatic assay for GHB in a forensic context.

PMID: 22722059 [PubMed - indexed for MEDLINE]