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One Cell, One Drop, One Click: Hybrid Microfluidics for Mammalian Single Cell Isolation.

Author(s): Samlali K, Ahmadi F, Quach ABV, Soffer G, Shih SCC

Small. 2020 Jul 23;:e2002400 Authors: Samlali K, Ahmadi F, Quach ABV, Soffer G, Shih SCC

Article GUID: 32705796
An integrated droplet-digital microfluidic system for on-demand droplet creation, mixing, incubation, and sorting.

Author(s): Ahmadi F, Samlali K, Vo PQN, Shih SCC

Lab Chip. 2019 01 29;19(3):524-535 Authors: Ahmadi F, Samlali K, Vo PQN, Shih SCC

Article GUID: 30633267
Title:One Cell, One Drop, One Click: Hybrid Microfluidics for Mammalian Single Cell Isolation.
Authors:Samlali K,  Ahmadi F,  Quach ABV,  Soffer G,  Shih SCC
Link:https://www.ncbi.nlm.nih.gov/pubmed/32705796
DOI:10.1002/smll.202002400
Category:Small
PMID:32705796
Dept Affiliation: BIOLOGY
1 Department of Electrical and Computer Engineering, Concordia University, Montréal, Québec, H3G 1M8, Canada.
2 Centre for Applied Synthetic Biology, Concordia University, Montréal, Québec, H4B 1R6, Canada.
3 Department of Biology, Concordia University, Montréal, Québec, H4B 1R6, Canada.

Description:

One Cell, One Drop, One Click: Hybrid Microfluidics for Mammalian Single Cell Isolation.

Small. 2020 Jul 23;:e2002400

Authors: Samlali K, Ahmadi F, Quach ABV, Soffer G, Shih SCC

Abstract

Generating a stable knockout cell line is a complex process that can take several months to complete. In this work, a microfluidic method that is capable of isolating single cells in droplets, selecting successful edited clones, and expansion of these isoclones is introduced. Using a hybrid microfluidics method, droplets in channels can be individually addressed using a co-planar electrode system. In the hybrid microfluidics device, it is shown that single cells can be trapped and subsequently encapsulate them on demand into pL-sized droplets. Furthermore, droplets containing single cells are either released, kept in the traps, or merged with other droplets by the application of an electric potential to the electrodes that is actuated through an in-house user interface. This high precision control is used to successfully sort and recover single isoclones to establish monoclonal cell lines, which is demonstrated with a heterozygous NCI-H1299 lung squamous cell population resulting from loss-of-function eGFP and RAF1 gene knockout transfections.

PMID: 32705796 [PubMed - as supplied by publisher]