Keyword search (3,448 papers available)


Auditory information enhances post-sensory visual evidence during rapid multisensory decision-making.

Author(s): Franzen L; Delis I; De Sousa G; Kayser C; Philiastides MG;

Despite recent progress in understanding multisensory decision-making, a conclusive mechanistic account of how the brain translates the relevant evidence into a decision is lacking. Specifically, it remains unclear whether perceptual improvements during rap...

Article GUID: 33116148
Dissociable mesolimbic dopamine circuits control responding triggered by alcohol-predictive discrete cues and contexts.

Author(s): Valyear MD, Glovaci I, Zaari A, Lahlou S, Trujillo-Pisanty I, Andrew Chapman C, Chaudhri N

Nat Commun. 2020 Jul 28;11(1):3764 Authors: Valyear MD, Glovaci I, Zaari A, Lahlou S, Trujillo-Pisanty I, Andrew Chapman C, Chaudhri N

Article GUID: 32724058
A yeast platform for high-level synthesis of tetrahydroisoquinoline alkaloids.

Author(s): Pyne ME, Kevvai K, Grewal PS, Narcross L, Choi B, Bourgeois L, Dueber JE, Martin VJJ

Nat Commun. 2020 Jul 03;11(1):3337 Authors: Pyne ME, Kevvai K, Grewal PS, Narcross L, Choi B, Bourgeois L, Dueber JE, Martin VJJ

Article GUID: 32620756
Evolving perspectives on the sources of the frequency-following response.

Author(s): Coffey EBJ, Nicol T, White-Schwoch T, Chandrasekaran B, Krizman J, Skoe E, Zatorre RJ, Kraus N

Nat Commun. 2019 Nov 06;10(1):5036 Authors: Coffey EBJ, Nicol T, White-Schwoch T, Chandrasekaran B, Krizman J, Skoe E, Zatorre RJ, Kraus N

Article GUID: 31695046
Bacterial variability in the mammalian gut captured by a single-cell synthetic oscillator.

Author(s): Riglar DT, Richmond DL, Potvin-Trottier L, Verdegaal AA, Naydich AD, Bakshi S, Leoncini E, Lyon LG, Paulsson J, Silver PA

Nat Commun. 2019 Oct 11;10(1):4665 Authors: Riglar DT, Richmond DL, Potvin-Trottier L, Verdegaal AA, Naydich AD, Bakshi S, Leoncini E, Lyon LG, Paulsson J, Silver PA

Article GUID: 31604953
Non-invasive in vivo hyperspectral imaging of the retina for potential biomarker use in Alzheimer's disease.

Author(s): Hadoux X, Hui F, Lim JKH, Masters CL, Pébay A, Chevalier S, Ha J, Loi S, Fowler CJ, Rowe C, Villemagne VL, Taylor EN, Fluke C, Soucy JP, Les...

Nat Commun. 2019 Sep 17;10(1):4227 Authors: Hadoux X, Hui F, Lim JKH, Masters CL, Pébay A, Chevalier S, Ha J, Loi S, Fowler CJ, Rowe C, Villemagne VL, Taylor EN, Fluke C, Soucy JP, Lesage F, ...

Article GUID: 31530809
Author Correction: Building a global alliance of biofoundries.

Author(s): Hillson N, Caddick M, Cai Y, Carrasco JA, Chang MW, Curach NC, Bell DJ, Feuvre RL, Friedman DC, Fu X, Gold ND, Herrgård MJ, Holowko MB, John...

Nat Commun. 2019 Jul 11;10(1):3132 Authors: Hillson N, Caddick M, Cai Y, Carrasco JA, Chang MW, Curach NC, Bell DJ, Feuvre RL, Friedman DC, Fu X, Gold ND, Herrgård MJ, Holowko MB, Johnson JR,...

Article GUID: 31296848
Neural correlates of two different types of extinction learning in the amygdala central nucleus.

Author(s): Iordanova MD, Deroche ML, Esber GR, Schoenbaum G

Nat Commun. 2016 08 17;7:12330 Authors: Iordanova MD, Deroche ML, Esber GR, Schoenbaum G

Article GUID: 27531638
The intralumenal fragment pathway mediates ESCRT-independent surface transporter down-regulation.

Author(s): McNally EK, Brett CL

Nat Commun. 2018 12 18;9(1):5358 Authors: McNally EK, Brett CL

Article GUID: 30560896
A platform for glycoengineering a polyvalent pneumococcal bioconjugate vaccine using E. coli as a host.

Author(s): Harding CM, Nasr MA, Scott NE, Goyette-Desjardins G, Nothaft H, Mayer AE, Chavez SM, Huynh JP, Kinsella RL, Szymanski CM, Stallings CL, Segu...

Nat Commun. 2019 02 21;10(1):891 Authors: Harding CM, Nasr MA, Scott NE, Goyette-Desjardins G, Nothaft H, Mayer AE, Chavez SM, Huynh JP, Kinsella RL, Szymanski CM, Stallings CL, Segura M, Feldman MF

Article GUID: 30792408
Reconstitution of a 10-gene pathway for synthesis of the plant alkaloid dihydrosanguinarine in Saccharomyces cerevisiae.

Author(s): Fossati E, Ekins A, Narcross L, Zhu Y, Falgueyret JP, Beaudoin GA, Facchini PJ, Martin VJ

Nat Commun. 2014;5:3283 Authors: Fossati E, Ekins A, Narcross L, Zhu Y, Falgueyret JP, Beaudoin GA, Facchini PJ, Martin VJ

Article GUID: 24513861
Giant electron-hole transport asymmetry in ultra-short quantum transistors.

Author(s): McRae AC, Tayari V, Porter JM, Champagne AR

Nat Commun. 2017 05 31;8:15491 Authors: McRae AC, Tayari V, Porter JM, Champagne AR

Article GUID: 28561024
Building a global alliance of biofoundries.

Author(s): Hillson N, Caddick M, Cai Y, Carrasco JA, Chang MW, Curach NC, Bell DJ, Le Feuvre R, Friedman DC, Fu X, Gold ND, Herrgård MJ, Holowko MB, Jo...

Nat Commun. 2019 05 09;10(1):2040 Authors: Hillson N, Caddick M, Cai Y, Carrasco JA, Chang MW, Curach NC, Bell DJ, Le Feuvre R, Friedman DC, Fu X, Gold ND, Herrgård MJ, Holowko MB, Johnson JR...

Article GUID: 31068573
Title:A platform for glycoengineering a polyvalent pneumococcal bioconjugate vaccine using E. coli as a host.
Authors:Harding CM,  Nasr MA,  Scott NE,  Goyette-Desjardins G,  Nothaft H,  Mayer AE,  Chavez SM,  Huynh JP,  Kinsella RL,  Szymanski CM,  Stallings CL,  Segura M,  Feldman MF
Link:https://www.ncbi.nlm.nih.gov/pubmed/30792408?dopt=Abstract
DOI:10.1038/s41467-019-08869-9
Category:Nat Commun
PMID:30792408
Dept Affiliation: BIOLOGY
1 VaxNewMo LLC, St. Louis, MO, 63108, USA. christian.harding@vaxnewmo.com.
2 Department of Biological Sciences, University of Alberta, Edmonton, AB, T6G 2R3, Canada.
3 Department of Biology, Centre for Applied Synthetic Biology, Concordia University, Montreal, QC, H4B 1R6, Canada.
4 Department of Microbiology and Immunology, Institute for Infection and Immunity, University of Melbourne at the Peter Doherty, Parkville, VIC, 3010, Australia.
5 Swine and Poultry Infectious Diseases Research Center, Faculty of Veterinary Medicine, University of Montreal, 3200 Sicotte Street, St-Hyacinthe, QC, J2S 2M2, Canada.
6 Department of Molecular Microbiology, Washington University School of Medicine, St Louis, MO, 63110, USA.
7 Department of Microbiology and Complex Carbohydrate Research Center, University of Georgia, Athens, GA, 30602, USA.
8 VaxNewMo LLC, St. Louis, MO, 63108, USA. mariofeldman@wustl.edu.
9 Department of Molecular Microbiology, Washington University School of Medicine, St Louis, MO, 63110, USA. mariofeldman@wustl.edu.

Description:

A platform for glycoengineering a polyvalent pneumococcal bioconjugate vaccine using E. coli as a host.

Nat Commun. 2019 02 21;10(1):891

Authors: Harding CM, Nasr MA, Scott NE, Goyette-Desjardins G, Nothaft H, Mayer AE, Chavez SM, Huynh JP, Kinsella RL, Szymanski CM, Stallings CL, Segura M, Feldman MF

Abstract

Chemical synthesis of conjugate vaccines, consisting of a polysaccharide linked to a protein, can be technically challenging, and in vivo bacterial conjugations (bioconjugations) have emerged as manufacturing alternatives. Bioconjugation relies upon an oligosaccharyltransferase to attach polysaccharides to proteins, but currently employed enzymes are not suitable for the generation of conjugate vaccines when the polysaccharides contain glucose at the reducing end, which is the case for ~75% of Streptococcus pneumoniae capsules. Here, we use an O-linking oligosaccharyltransferase to generate a polyvalent pneumococcal bioconjugate vaccine with polysaccharides containing glucose at their reducing end. In addition, we show that different vaccine carrier proteins can be glycosylated using this system. Pneumococcal bioconjugates are immunogenic, protective and rapidly produced within E. coli using recombinant techniques. These proof-of-principle experiments establish a platform to overcome limitations of other conjugating enzymes enabling the development of bioconjugate vaccines for many important human and animal pathogens.

PMID: 30792408 [PubMed - indexed for MEDLINE]