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Author(s): Sgro M; Reid ID; Arentshorst M; Ram AFJ; Tsang A;
Many microorganisms are able to use plant-derived aromatic and cyclic compounds like the common plant secondary metabolite quinic acid as carbon and energy sources. In fungi, three enzymatic steps convert quinic acid into the common intermediate protocatech...
Article GUID: 40853219
Author(s): Semper C; Pham TTM; Ram S; Palys S; Evdokias G; Ouedraogo JP; Moisan MC; Geoffrion N; Reid I; Di Falco M; Bailey Z; Tsang A; Benoit-Gelber I...
Genomics analysis confirmed the status of filamentous fungi as a rich source of novel secondary metabolites; however, the discovery of these compounds is hampered by the cryptic nature of their bio...
Article GUID: 40852424
Author(s): Raheja Y; Singh V; Gaur VK; Sharma G; Tsang A; Chadha BS;
GH7 cellobiohydrolases (CBH1s) are essential for depolymerizing crystalline cellulose, yet the hypercellulolytic thermophile Rasamsonia emersonii secretes them only in low amounts, leaving a gap in its native enzyme cocktail. To see whether a cognate CBH1 c...
Article GUID: 40622460
Author(s): Raheja Y; Singh V; Gaur VK; Tsang A; Chadha BS;
In this study, two thermostable endoglucanases (Rem_GH5EG and Rem_GH7EG) from Rasamsonia emersonii were heterologously expressed in Pichia pastoris and characterized to evaluate their potential for industrial biomass saccharification. Rem_GH5EG demonstrated...
Article GUID: 40418313
| Title: | Fortifying the Rasamsonia emersonii secretome with recombinant cellobiohydrolase (GH7) for efficient biomass saccharification |
| Authors: | Raheja Y, Singh V, Gaur VK, Sharma G, Tsang A, Chadha BS, |
| Link: | https://pubmed.ncbi.nlm.nih.gov/40622460/ |
| DOI: | 10.1007/s11274-025-04473-w |
| Category: | |
| PMID: | 40622460 |
| Dept Affiliation: | GENOMICS
1 Department of Microbiology, Guru Nanak Dev University, Amritsar, Punjab, 143005, India. yashikaraheja27@gmail.com. 2 School of Energy and Chemical Engineering, Ulsan National Institute of Science and Technology, Ulsan, 44919, Republic of Korea. yashikaraheja27@gmail.com. 3 Department of Microbiology, Guru Nanak Dev University, Amritsar, Punjab, 143005, India. 4 School of Energy and Chemical Engineering, Ulsan National Institute of Science and Technology, Ulsan, 44919, Republic of Korea. 5 Amity Institute of Biotechnology, Amity University Uttar Pradesh, Noida, India. 6 Department of Microbiology, DAV university Jalandhar, Jalandhar, Punjab, 144012, India. 7 Center for Structural and Functional Genomics, Concordia University, 7141 Sherbrooke Street West, Montreal, QC, H4B 1R6, Canada. |
Description: |
GH7 cellobiohydrolases (CBH1s) are essential for depolymerizing crystalline cellulose, yet the hypercellulolytic thermophile Rasamsonia emersonii secretes them only in low amounts, leaving a gap in its native enzyme cocktail. To see whether a cognate CBH1 could fill this gap and how it stacks up against the industrial workhorse strain Trichoderma reesei Cel7A, we codon optimized the R. emersonii gene (Rem_GH7CBHI), expressed it in Pichia pastoris and purified the recombinant enzyme for structural and functional analysis. The 57 kDa protein retains the canonical GH7 ßsandwich tunnel, but an AlaforTyr substitution leaves the channel more open than that of T. reesei, potentially easing substrate entry. Consistent with this architecture, Rem_GH7CBHI binds cellotriose tightly and exhibits a low K? of 0.25 mM. Biochemical characterization revealed the optimal activity at pH 5.0, 60 °C and retaining about 60% activity after 1 h at 70 °C. Adding Rem_GH7CBHI together with an endogenous AA9 LPMO to the native R. emersonii secretome (M36) boosted saccharification of steamacidpretreated rice straw to levels comparable with commercial Cellic CTec3 at the same total protein loading. These results position Rem_GH7CBHI as a thermostable, highaffinity alternative to T. reesei Cel7A and a costeffective addition to tailored enzyme cocktails for highsolids lignocellulose biorefineries. |
