Keyword search (4,163 papers available)

"Darlington PJ" Authored Publications:

Title Authors PubMed ID
1 Nicotine Suppresses Human Memory Th Cell Subsets With Preferential Effects on Central Memory Th Cells in an α7 Nicotinic Acetylcholine Receptor-Dependent Manner Gholizadeh F; Hajiaghayi M; Rahbari N; Choi JS; Heidt S; Como A; Kazerouni M; Kargar M; Pinard-LaRoche A; Shih SCC; Darlington PJ; 41928597
SOH
2 Nebivolol prevents exhausted T cells and enhances cytotoxicity against MCF-7 breast cancer cells in a β2-adrenergic receptor-dependent manner Hajiaghayi M; Gholizadeh F; Rahbari N; Emamnia N; Shih SCC; Darlington PJ; 41906691
SOH
3 Modulatory effects of M3 muscarinic acetylcholine receptor on inflammatory profiles of human memory T helper cells Gholizadeh F; Hajiaghayi M; Choi JS; Little SR; Rahbari N; Kargar M; Brotto K; Han E; Shih SCC; Darlington PJ; 40405417
BIOLOGY
4 A Digital Microfluidic Platform for the Microscale Production of Functional Immune Cell Therapies Little SR; Rahbari N; Hajiaghayi M; Gholizadeh F; Cloarec-Ung FM; Phillips J; Sinha H; Hirukawa A; Knapp DJHF; Darlington PJ; Shih SCC; 40390294
BIOLOGY
5 Immunomodulation of human T cells by microbubble-mediated focused ultrasound Baez A; Singh D; He S; Hajiaghayi M; Gholizadeh F; Darlington PJ; Helfield B; 39502696
BIOLOGY
6 The β2-adrenergic biased agonist nebivolol inhibits the development of Th17 and the response of memory Th17 cells in an NF-κB-dependent manner Hajiaghayi M; Gholizadeh F; Han E; Little SR; Rahbari N; Ardila I; Lopez Naranjo C; Tehranimeh K; Shih SCC; Darlington PJ; 39445009
BIOLOGY
7 The β2-adrenergic receptor agonist terbutaline upregulates T helper-17 cells in a protein kinase A-dependent manner Carvajal Gonczi CM; Hajiaghayi M; Gholizadeh F; Xavier Soares MA; Touma F; Lopez Naranjo C; Rios AJ; Pozzebon C; Daigneault T; Burchell-Reyes K; Darlington PJ; 37438188
PERFORM
8 Genetic Screening of Candida albicans Inactivation Mutants Identifies New Genes Involved in Macrophage-Fungal Cell Interactions Godoy P; Darlington PJ; Whiteway M; 35450285
PERFORM
9 Elevated Heart Rate and Pain During a Cold Pressor Test Correlates to Pain Catastrophizing Kakon G; Mohamadi AK; Levtova N; Maurice-Ventouris MEI; Benoit EA; Chouchou F; Darlington PJ; Dover G; 34453652
PERFORM
10 Association Between Pain Catastrophizing and Pain and Cardiovascular Changes During a Cold-Pressor Test in Athletes Lentini M; Scalia J; Lebel FB; Touma F; Jhajj A; Darlington PJ; Dover G; 34000018
PERFORM
11 Pain catastrophizing in athletes correlates with pain and cardiovascular changes during a painful cold pressor test Matylda L; Joseph S; Frédérike BL; Fadi T; Aneet J; Darlington PJ; Dover G; 33150380
PERFORM
12 Human Mesenchymal Stem Cells Impact Th17 and Th1 Responses Through a Prostaglandin E2 and Myeloid-Dependent Mechanism. Rozenberg A, Rezk A, Boivin MN, Darlington PJ, Nyirenda M, Li R, Jalili F, Winer R, Artsy EA, Uccelli A, Reese JS, Planchon SM, Cohen JA, Bar-Or A 27400792
HKAP
13 Comparative morphology and phagocytic capacity of primary human adult microglia with time-lapse imaging. Levtova N, Healy LM, Gonczi CMC, Stopnicki B, Blain M, Kennedy TE, Moore CS, Antel JP, Darlington PJ 28606377
PERFORM
14 Detecting glycogen in peripheral blood mononuclear cells with periodic acid schiff staining. Tabatabaei Shafiei M, Carvajal Gonczi CM, Rahman MS, East A, François J, Darlington PJ 25548935
PERFORM
15 Reciprocal modulation of helper Th1 and Th17 cells by the β2-adrenergic receptor agonist drug terbutaline. Carvajal Gonczi CM, Tabatabaei Shafiei M, East A, Martire E, Maurice-Ventouris MHI, Darlington PJ 28710773
PERFORM
16 Natural Killer Cells Regulate Th17 Cells After Autologous Hematopoietic Stem Cell Transplantation for Relapsing Remitting Multiple Sclerosis. Darlington PJ, Stopnicki B, Touil T, Doucet JS, Fawaz L, Roberts ME, Boivin MN, Arbour N, Freedman MS, Atkins HL, Bar-Or A 29867923
PERFORM
17 Helper CD4 T cells expressing granzyme B cause glial fibrillary acidic protein fragmentation in astrocytes in an MHCII-independent manner. Stopnicki B, Blain M, Cui QL, Kennedy TE, Antel JP, Healy LM, Darlington PJ 30444064
PERFORM

 

Title:Helper CD4 T cells expressing granzyme B cause glial fibrillary acidic protein fragmentation in astrocytes in an MHCII-independent manner.
Authors:Stopnicki BBlain MCui QLKennedy TEAntel JPHealy LMDarlington PJ
Link:https://www.ncbi.nlm.nih.gov/pubmed/30444064?dopt=Abstract
DOI:10.1002/glia.23503
Publication:Glia
Keywords:GFAPT cellastrocytecaspasecytoskeletongranzyme Bneuroinflammation
PMID:30444064 Category:Glia Date Added:2019-04-15
Dept Affiliation: PERFORM
1 Department of Exercise Science, Department of Biology, PERFORM Centre, Concordia University, Montréal, Quebec, Canada.
2 Department of Neurology and Neurosurgery, Montreal Neurological Institute, and McGill University, Montreal, Quebec, Canada.
3 Neuroimmunology Unit, McGill University, Montréal, Quebec, Canada.

Description:

Helper CD4 T cells expressing granzyme B cause glial fibrillary acidic protein fragmentation in astrocytes in an MHCII-independent manner.

Glia. 2019 04;67(4):582-593

Authors: Stopnicki B, Blain M, Cui QL, Kennedy TE, Antel JP, Healy LM, Darlington PJ

Abstract

During inflammatory processes of the central nervous system, helper T cells have the capacity to cross the blood-brain barrier and injure or kill neural cells through cytotoxic mechanisms. Glial fibrillary acidic protein (GFAP) is an intermediate filament protein that is part of the astrocyte cytoskeleton that can become fragmented in neuroinflammatory conditions. The mechanism of action by which helper T cells with cytotoxic properties injure astrocytes is not completely understood. Primary human astrocytes were obtained from fetal brain tissue. Human helper (CD4+ ) T cells were isolated from peripheral blood mononuclear cells and activated with the superantigen staphylococcal enterotoxin E (SEE). Granzyme B was detected by enzyme linked immunosorbent assay and intracellular flow cytometry. GFAP fragmentation was monitored by western blotting. Cell death was monitored by lactic acid dehydrogenase release and terminal biotin-dUTP nick labeling (TUNEL). Astrocyte migration was monitored by scratch assay. Adult human oligodendrocytes were cultured with sublethally injured astrocytes to determine support function. Helper T cells activated with SEE expressed granzyme B but not perforin. Helper T cells released granzyme B upon contact with astrocytes and caused GFAP fragmentation in a caspase-dependent, MHCII-independent manner. Sublethally injured astrocytes were not apoptotic; however, their processes were thin and elongated, their migration was attenuated, and their ability to support oligodendrocytes was reduced in vitro. Helper T cells can release granzyme B causing sublethal injury to astrocytes, which compromises the supportive functions of astrocytes. Blocking these pathways may lead to improved resolution of neuroinflammatory lesions.

PMID: 30444064 [PubMed - in process]





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