BookR: School of Health Core Facilities Booking
PubMed Publications| Keyword search (4,164 papers available) |  |
"Darlington PJ" Authored Publications:
| Title | Authors | PubMed ID | |
|---|---|---|---|
| 1 | Nicotine Suppresses Human Memory Th Cell Subsets With Preferential Effects on Central Memory Th Cells in an α7 Nicotinic Acetylcholine Receptor-Dependent Manner | Gholizadeh F; Hajiaghayi M; Rahbari N; Choi JS; Heidt S; Como A; Kazerouni M; Kargar M; Pinard-LaRoche A; Shih SCC; Darlington PJ; | 41928597 SOH |
| 2 | Nebivolol prevents exhausted T cells and enhances cytotoxicity against MCF-7 breast cancer cells in a β2-adrenergic receptor-dependent manner | Hajiaghayi M; Gholizadeh F; Rahbari N; Emamnia N; Shih SCC; Darlington PJ; | 41906691 SOH |
| 3 | Modulatory effects of M3 muscarinic acetylcholine receptor on inflammatory profiles of human memory T helper cells | Gholizadeh F; Hajiaghayi M; Choi JS; Little SR; Rahbari N; Kargar M; Brotto K; Han E; Shih SCC; Darlington PJ; | 40405417 BIOLOGY |
| 4 | A Digital Microfluidic Platform for the Microscale Production of Functional Immune Cell Therapies | Little SR; Rahbari N; Hajiaghayi M; Gholizadeh F; Cloarec-Ung FM; Phillips J; Sinha H; Hirukawa A; Knapp DJHF; Darlington PJ; Shih SCC; | 40390294 BIOLOGY |
| 5 | Immunomodulation of human T cells by microbubble-mediated focused ultrasound | Baez A; Singh D; He S; Hajiaghayi M; Gholizadeh F; Darlington PJ; Helfield B; | 39502696 BIOLOGY |
| 6 | The β2-adrenergic biased agonist nebivolol inhibits the development of Th17 and the response of memory Th17 cells in an NF-κB-dependent manner | Hajiaghayi M; Gholizadeh F; Han E; Little SR; Rahbari N; Ardila I; Lopez Naranjo C; Tehranimeh K; Shih SCC; Darlington PJ; | 39445009 BIOLOGY |
| 7 | The β2-adrenergic receptor agonist terbutaline upregulates T helper-17 cells in a protein kinase A-dependent manner | Carvajal Gonczi CM; Hajiaghayi M; Gholizadeh F; Xavier Soares MA; Touma F; Lopez Naranjo C; Rios AJ; Pozzebon C; Daigneault T; Burchell-Reyes K; Darlington PJ; | 37438188 PERFORM |
| 8 | Genetic Screening of Candida albicans Inactivation Mutants Identifies New Genes Involved in Macrophage-Fungal Cell Interactions | Godoy P; Darlington PJ; Whiteway M; | 35450285 PERFORM |
| 9 | Elevated Heart Rate and Pain During a Cold Pressor Test Correlates to Pain Catastrophizing | Kakon G; Mohamadi AK; Levtova N; Maurice-Ventouris MEI; Benoit EA; Chouchou F; Darlington PJ; Dover G; | 34453652 PERFORM |
| 10 | Association Between Pain Catastrophizing and Pain and Cardiovascular Changes During a Cold-Pressor Test in Athletes | Lentini M; Scalia J; Lebel FB; Touma F; Jhajj A; Darlington PJ; Dover G; | 34000018 PERFORM |
| 11 | Pain catastrophizing in athletes correlates with pain and cardiovascular changes during a painful cold pressor test | Matylda L; Joseph S; Frédérike BL; Fadi T; Aneet J; Darlington PJ; Dover G; | 33150380 PERFORM |
| 12 | Human Mesenchymal Stem Cells Impact Th17 and Th1 Responses Through a Prostaglandin E2 and Myeloid-Dependent Mechanism. | Rozenberg A, Rezk A, Boivin MN, Darlington PJ, Nyirenda M, Li R, Jalili F, Winer R, Artsy EA, Uccelli A, Reese JS, Planchon SM, Cohen JA, Bar-Or A | 27400792 HKAP |
| 13 | Comparative morphology and phagocytic capacity of primary human adult microglia with time-lapse imaging. | Levtova N, Healy LM, Gonczi CMC, Stopnicki B, Blain M, Kennedy TE, Moore CS, Antel JP, Darlington PJ | 28606377 PERFORM |
| 14 | Detecting glycogen in peripheral blood mononuclear cells with periodic acid schiff staining. | Tabatabaei Shafiei M, Carvajal Gonczi CM, Rahman MS, East A, François J, Darlington PJ | 25548935 PERFORM |
| 15 | Reciprocal modulation of helper Th1 and Th17 cells by the β2-adrenergic receptor agonist drug terbutaline. | Carvajal Gonczi CM, Tabatabaei Shafiei M, East A, Martire E, Maurice-Ventouris MHI, Darlington PJ | 28710773 PERFORM |
| 16 | Natural Killer Cells Regulate Th17 Cells After Autologous Hematopoietic Stem Cell Transplantation for Relapsing Remitting Multiple Sclerosis. | Darlington PJ, Stopnicki B, Touil T, Doucet JS, Fawaz L, Roberts ME, Boivin MN, Arbour N, Freedman MS, Atkins HL, Bar-Or A | 29867923 PERFORM |
| 17 | Helper CD4 T cells expressing granzyme B cause glial fibrillary acidic protein fragmentation in astrocytes in an MHCII-independent manner. | Stopnicki B, Blain M, Cui QL, Kennedy TE, Antel JP, Healy LM, Darlington PJ | 30444064 PERFORM |
| Title: | The β2-adrenergic receptor agonist terbutaline upregulates T helper-17 cells in a protein kinase A-dependent manner | ||||
| Authors: | Carvajal Gonczi CM, Hajiaghayi M, Gholizadeh F, Xavier Soares MA, Touma F, Lopez Naranjo C, Rios AJ, Pozzebon C, Daigneault T, Burchell-Reyes K, Darlington PJ | ||||
| Link: | https://pubmed.ncbi.nlm.nih.gov/37438188/ | ||||
| DOI: | 10.1016/j.humimm.2023.06.007 | ||||
| Publication: | Human immunology | ||||
| Keywords: | Beta-2-adrenergic receptor; CD4; IL-17A; Memory T cells; Terbutaline; | ||||
| PMID: | 37438188 | Category: | Date Added: | 2023-07-13 | |
| Dept Affiliation: |
PERFORM
1 Department of Biology, PERFORM Centre, Concordia University, Montreal, Quebec, Canada. 2 Department of Psychology, PERFORM Centre, Concordia University, Montreal, Quebec, Canada. 3 Department of Chemistry and Biochemistry, PERFORM Centre, Concordia University, Montreal, Quebec, Canada. 4 Department of Biology, PERFORM Centre, Concordia University, Montreal, Quebec, Canada; Department of Psychology, PERFORM Centre, Concordia University, Montreal, Quebec, Canada; Department of Health, Kinesiology & Applied Physiology, PERFORM Centre, Montreal, Quebec, Canada. Electronic address: peter.darlington@concordia.ca. |
||||
Description: |
Background: T helper 17 (Th17) cells produce IL-17A cytokine and can exacerbate autoimmune diseases and asthma. The ß2 adrenergic receptor is a g protein-coupled receptor that induces cAMP second messenger pathways. We tested the hypothesis that terbutaline, a ß2-adrenergic receptor-specific agonist, promotes IL-17 secretion by memory Th17 cells in a cAMP and PKA-dependent manner. Methods: Venous peripheral blood mononuclear cells (PBMC) from healthy human participants were activated with anti-CD3 and anti-CD28 antibodies. Secreted IL-17A was measured by enzyme linked immunosorbent assay, intracellular IL-17A, and ROR? were measured using flow cytometry, and RORC by qPCR. Memory CD3+CD4+CD45RA-CD45RO+ T cells were obtained by immunomagnetic negative selection and activated with tri-antibody complex CD3/CD28/CD2. Secreted IL-17A, intracellular IL-17A, RORC were measured, and phosphorylated-serine133-CREB was measured by western blotting memory Th cells. Results: Terbutaline increased IL-17A (p < 0.001), IL-17A+ cells (p < 0.05), and RORC in activated PBMC and memory Th cells. The PKA inhibitors H89 (p < 0.001) and Rp-cAMP (p < 0.01) abrogated the effects of terbutaline on IL-17A secretion in PBMC and memory T cells. Rolipram increased IL-17A (p < 0.01) to a similar extent as terbutaline. P-Ser133-CREB was increased by terbutaline (p < 0.05) in memory T cells. Conclusion: Terbutaline augments memory Th17 cells in lymphocytes from healthy participants. This could exacerbate autoimmune diseases or asthma, in cases where Th17 cells are considered to be pro-inflammatory. |
