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PubMed Publications| Keyword search (4,163 papers available) |  |
"Darlington PJ" Authored Publications:
| Title | Authors | PubMed ID | |
|---|---|---|---|
| 1 | Nicotine Suppresses Human Memory Th Cell Subsets With Preferential Effects on Central Memory Th Cells in an α7 Nicotinic Acetylcholine Receptor-Dependent Manner | Gholizadeh F; Hajiaghayi M; Rahbari N; Choi JS; Heidt S; Como A; Kazerouni M; Kargar M; Pinard-LaRoche A; Shih SCC; Darlington PJ; | 41928597 SOH |
| 2 | Nebivolol prevents exhausted T cells and enhances cytotoxicity against MCF-7 breast cancer cells in a β2-adrenergic receptor-dependent manner | Hajiaghayi M; Gholizadeh F; Rahbari N; Emamnia N; Shih SCC; Darlington PJ; | 41906691 SOH |
| 3 | Modulatory effects of M3 muscarinic acetylcholine receptor on inflammatory profiles of human memory T helper cells | Gholizadeh F; Hajiaghayi M; Choi JS; Little SR; Rahbari N; Kargar M; Brotto K; Han E; Shih SCC; Darlington PJ; | 40405417 BIOLOGY |
| 4 | A Digital Microfluidic Platform for the Microscale Production of Functional Immune Cell Therapies | Little SR; Rahbari N; Hajiaghayi M; Gholizadeh F; Cloarec-Ung FM; Phillips J; Sinha H; Hirukawa A; Knapp DJHF; Darlington PJ; Shih SCC; | 40390294 BIOLOGY |
| 5 | Immunomodulation of human T cells by microbubble-mediated focused ultrasound | Baez A; Singh D; He S; Hajiaghayi M; Gholizadeh F; Darlington PJ; Helfield B; | 39502696 BIOLOGY |
| 6 | The β2-adrenergic biased agonist nebivolol inhibits the development of Th17 and the response of memory Th17 cells in an NF-κB-dependent manner | Hajiaghayi M; Gholizadeh F; Han E; Little SR; Rahbari N; Ardila I; Lopez Naranjo C; Tehranimeh K; Shih SCC; Darlington PJ; | 39445009 BIOLOGY |
| 7 | The β2-adrenergic receptor agonist terbutaline upregulates T helper-17 cells in a protein kinase A-dependent manner | Carvajal Gonczi CM; Hajiaghayi M; Gholizadeh F; Xavier Soares MA; Touma F; Lopez Naranjo C; Rios AJ; Pozzebon C; Daigneault T; Burchell-Reyes K; Darlington PJ; | 37438188 PERFORM |
| 8 | Genetic Screening of Candida albicans Inactivation Mutants Identifies New Genes Involved in Macrophage-Fungal Cell Interactions | Godoy P; Darlington PJ; Whiteway M; | 35450285 PERFORM |
| 9 | Elevated Heart Rate and Pain During a Cold Pressor Test Correlates to Pain Catastrophizing | Kakon G; Mohamadi AK; Levtova N; Maurice-Ventouris MEI; Benoit EA; Chouchou F; Darlington PJ; Dover G; | 34453652 PERFORM |
| 10 | Association Between Pain Catastrophizing and Pain and Cardiovascular Changes During a Cold-Pressor Test in Athletes | Lentini M; Scalia J; Lebel FB; Touma F; Jhajj A; Darlington PJ; Dover G; | 34000018 PERFORM |
| 11 | Pain catastrophizing in athletes correlates with pain and cardiovascular changes during a painful cold pressor test | Matylda L; Joseph S; Frédérike BL; Fadi T; Aneet J; Darlington PJ; Dover G; | 33150380 PERFORM |
| 12 | Human Mesenchymal Stem Cells Impact Th17 and Th1 Responses Through a Prostaglandin E2 and Myeloid-Dependent Mechanism. | Rozenberg A, Rezk A, Boivin MN, Darlington PJ, Nyirenda M, Li R, Jalili F, Winer R, Artsy EA, Uccelli A, Reese JS, Planchon SM, Cohen JA, Bar-Or A | 27400792 HKAP |
| 13 | Comparative morphology and phagocytic capacity of primary human adult microglia with time-lapse imaging. | Levtova N, Healy LM, Gonczi CMC, Stopnicki B, Blain M, Kennedy TE, Moore CS, Antel JP, Darlington PJ | 28606377 PERFORM |
| 14 | Detecting glycogen in peripheral blood mononuclear cells with periodic acid schiff staining. | Tabatabaei Shafiei M, Carvajal Gonczi CM, Rahman MS, East A, François J, Darlington PJ | 25548935 PERFORM |
| 15 | Reciprocal modulation of helper Th1 and Th17 cells by the β2-adrenergic receptor agonist drug terbutaline. | Carvajal Gonczi CM, Tabatabaei Shafiei M, East A, Martire E, Maurice-Ventouris MHI, Darlington PJ | 28710773 PERFORM |
| 16 | Natural Killer Cells Regulate Th17 Cells After Autologous Hematopoietic Stem Cell Transplantation for Relapsing Remitting Multiple Sclerosis. | Darlington PJ, Stopnicki B, Touil T, Doucet JS, Fawaz L, Roberts ME, Boivin MN, Arbour N, Freedman MS, Atkins HL, Bar-Or A | 29867923 PERFORM |
| 17 | Helper CD4 T cells expressing granzyme B cause glial fibrillary acidic protein fragmentation in astrocytes in an MHCII-independent manner. | Stopnicki B, Blain M, Cui QL, Kennedy TE, Antel JP, Healy LM, Darlington PJ | 30444064 PERFORM |
| Title: | Detecting glycogen in peripheral blood mononuclear cells with periodic acid schiff staining. | ||||
| Authors: | Tabatabaei Shafiei M, Carvajal Gonczi CM, Rahman MS, East A, François J, Darlington PJ | ||||
| Link: | www.ncbi.nlm.nih.gov/pubmed/25548935?dopt=Abstract | ||||
| Publication: | |||||
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| PMID: | 25548935 | Category: | J Vis Exp | Date Added: | 2019-04-15 |
| Dept Affiliation: |
PERFORM
1 Department of Biology, Centre for Structural and Functional Genomics, PERFORM Centre, Concordia University. 2 Department of Chemistry and Biochemistry, Centre for Structural and Functional Genomics, PERFORM Centre, Concordia University. 3 Department of Exercise Science, Centre for Structural and Functional Genomics, PERFORM Centre, Concordia University. 4 Department of Biology, Centre for Structural and Functional Genomics, PERFORM Centre, Concordia University; Department of Exercise Science, Centre for Structural and Functional Genomics, PERFORM Centre, Concordia University; peter.darlington@concordia.ca. |
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Description: |
Detecting glycogen in peripheral blood mononuclear cells with periodic acid schiff staining. J Vis Exp. 2014 Dec 23;(94): Authors: Tabatabaei Shafiei M, Carvajal Gonczi CM, Rahman MS, East A, François J, Darlington PJ Abstract Periodic acid Schiff (PAS) staining is an immunohistochemical technique used on muscle biopsies and as a diagnostic tool for blood samples. Polysaccharides such as glycogen, glycoproteins, and glycolipids stain bright magenta making it easy to enumerate positive and negative cells within the tissue. In muscle cells PAS staining is used to determine the glycogen content in different types of muscle cells, while in blood cell samples PAS staining has been explored as a diagnostic tool for a variety of conditions. Blood contains a proportion of white blood cells that belong to the immune system. The notion that cells of the immune system possess glycogen and use it as an energy source has not been widely explored. Here, we describe an adapted version of the PAS staining protocol that can be applied on peripheral blood mononuclear immune cells from human venous blood. Small cells with PAS-positive granules and larger cells with diffuse PAS staining were observed. Treatment of samples with amylase abrogates these patterns confirming the specificity of the stain. An alternate technique based on enzymatic digestion confirmed the presence and amount of glycogen in the samples. This protocol is useful for hematologists or immunologists studying polysaccharide content in blood-derived lymphocytes. PMID: 25548935 [PubMed - indexed for MEDLINE] |
