Keyword search (3,619 papers available)


A reliable, reproducible flow cytometry protocol for immune cell quantification in human adipose tissue.

Author(s): Delaney KZ, Dam V, Murphy J, Morais JA, Denis R, Atlas H, Pescarus R, Garneau PY, Santosa S...

The ability to accurately identify and quantify immune cell populations within adipose tissue is important in understanding the role of immune cells in metabolic disease risk. Flow cytometry is the...

Article GUID: 32926866
Acetyl-CoA regulation, OXPHOS integrity and leptin level are different in females with different onsets of obesity.

Author(s): Tam BT, Murphy J, Khor N, Morais JA, Santosa S

Although childhood-onset obesity (CO) and adult-onset obesity (AO) are known to lead to distinctive clinical manifestations and disease risks, the fundamental differences between them are largely unclear. The aim of the current study is to investigate the f...

Article GUID: 32808657
Sex differences in the relationship between dietary pattern adherence and cognitive function among older adults: findings from the NuAge study.

Author(s): D'Amico D, Parrott MD, Greenwood CE, Ferland G, Gaudreau P, Belleville S, Laurin D, Anderson ND, Kergoat MJ, Morais JA, Presse N, Fiocco AJ,

Nutr J. 2020 Jun 20;19(1):58 Authors: D'Amico D, Parrott MD, Greenwood CE, Ferland G, Gaudreau P, Belleville S, Laurin D, Anderson ND, Kergoat MJ, Morais JA, Presse N, Fiocco AJ

Article GUID: 32563260
Obesity and ageing: Two sides of the same coin.

Author(s): Tam BT, Morais JA, Santosa S

Obes Rev. 2020 Feb 05;: Authors: Tam BT, Morais JA, Santosa S

Article GUID: 32020741
Guidelines for Gait Assessments in the Canadian Consortium on Neurodegeneration in Aging (CCNA).

Author(s): Cullen S, Montero-Odasso M, Bherer L, Almeida Q, Fraser S, Muir-Hunter S, Li K, Liu-Ambrose T, McGibbon CA, McIlroy W, Middleton LE, Sarquis...

Can Geriatr J. 2018 Jun;21(2):157-165 Authors: Cullen S, Montero-Odasso M, Bherer L, Almeida Q, Fraser S, Muir-Hunter S, Li K, Liu-Ambrose T, McGibbon CA, McIlroy W, Middleton LE, Sarquis-Adamson ...

Article GUID: 29977431
SYNERGIC TRIAL (SYNchronizing Exercises, Remedies in Gait and Cognition) a multi-Centre randomized controlled double blind trial to improve gait and cognition in mild cognitive impairment.

Author(s): Montero-Odasso M, Almeida QJ, Burhan AM, Camicioli R, Doyon J, Fraser S, Li K, Liu-Ambrose T, Middleton L, Muir-Hunter S, McIlroy W, Morais ...

BMC Geriatr. 2018 04 16;18(1):93 Authors: Montero-Odasso M, Almeida QJ, Burhan AM, Camicioli R, Doyon J, Fraser S, Li K, Liu-Ambrose T, Middleton L, Muir-Hunter S, McIlroy W, Morais JA, Pieruccini...

Article GUID: 29661156
Consensus on Shared Measures of Mobility and Cognition: From the Canadian Consortium on Neurodegeneration in Aging (CCNA).

Author(s): Montero-Odasso M, Almeida QJ, Bherer L, Burhan AM, Camicioli R, Doyon J, Fraser S, Muir-Hunter S, Li KZH, Liu-Ambrose T, McIlroy W, Middleto...

J Gerontol A Biol Sci Med Sci. 2019 May 16;74(6):897-909 Authors: Montero-Odasso M, Almeida QJ, Bherer L, Burhan AM, Camicioli R, Doyon J, Fraser S, Muir-Hunter S, Li KZH, Liu-Ambrose T, McIlroy W...

Article GUID: 30101279
Use of routinely available clinical, nutritional, and functional criteria to classify cachexia in advanced cancer patients.

Author(s): Vigano AAL, Morais JA, Ciutto L, Rosenthall L, di Tomasso J, Khan S, Olders H, Borod M, Kilgour RD

Clin Nutr. 2017 10;36(5):1378-1390 Authors: Vigano AAL, Morais JA, Ciutto L, Rosenthall L, di Tomasso J, Khan S, Olders H, Borod M, Kilgour RD

Article GUID: 27793524
Intra-Abdominal Adipose Tissue Quantification by Alternative Versus Reference Methods: A Systematic Review and Meta-Analysis.

Author(s): Murphy J, Bacon SL, Morais JA, Tsoukas MA, Santosa S

Obesity (Silver Spring). 2019 May 27;: Authors: Murphy J, Bacon SL, Morais JA, Tsoukas MA, Santosa S

Article GUID: 31131996
Title:A reliable, reproducible flow cytometry protocol for immune cell quantification in human adipose tissue.
Authors:Delaney KZ,  Dam V,  Murphy J,  Morais JA,  Denis R,  Atlas H,  Pescarus R,  Garneau PY,  Santosa S
Link:https://www.ncbi.nlm.nih.gov/pubmed/32926866
DOI:10.1016/j.ab.2020.113951
Category:Anal Biochem
PMID:32926866
Dept Affiliation: PERFORM
1 Department of Health, Kinesiology, and Applied Physiology, Concordia University, 7141 Sherbrooke St W, Montreal, QC, H4B 1R6, Canada; Metabolism, Obesity, Nutrition Lab, PERFORM Centre, Concordia University, 7200 Sherbrooke St W, Montreal, QC, H4B 1R6, Canada; Centre de Recherche - Axe Maladies Chroniques, Centre Intégré Universitaire de Santé et de Services Sociaux Du Nord-de-l'Ile-de-Montreal, Hôpital Du Sacré-Coeur de Montreal, 5400 Boul Gouin O, Montréal, QC, H4J 1C5, Canada.
2 Department of Health, Kinesiology, and Applied Physiology, Concordia University, 7141 Sherbrooke St W, Montreal, QC, H4B 1R6, Canada; Division of Geriatric Medicine, McGill University Health Centre - Royal Victoria Hospital, 1001, Décarie, Montreal, Québec, H4A 3J1, Canada.
3 Departement Du Chirurgie, Hôpital Du Sacré-Coeur de Montréal, 5400 Boul Gouin O, Montréal, QC, H4J 1C5, Canada.
4 Department of Health, Kinesiology, and Applied Physiology, Concordia University, 7141 Sherbrooke St W, Montreal, QC, H4B 1R6, Canada; Metabolism, Obesity, Nutrition Lab, PERFORM Centre, Concordia University, 7200 Sherbrooke St W, Montreal, QC, H4B 1R6, Canada; Centre de Recherche - Axe Maladies Chroniques, Centre Intégré Universitaire de Santé et de Services Sociaux Du Nord-de-l'Ile-de-Montreal, Hôpital Du Sacré-Coeur de Montreal, 5400 Boul Gouin O, Montréal, QC, H4J 1C5, Canada. Electronic address: s.santosa@concordia.ca.

Description:

A reliable, reproducible flow cytometry protocol for immune cell quantification in human adipose tissue.

Anal Biochem. 2020 Sep 11; :113951

Authors: Delaney KZ, Dam V, Murphy J, Morais JA, Denis R, Atlas H, Pescarus R, Garneau PY, Santosa S

Abstract

The ability to accurately identify and quantify immune cell populations within adipose tissue is important in understanding the role of immune cells in metabolic disease risk. Flow cytometry is the gold standard method for immune cell quantification. However, quantification of immune cells from adipose tissue presents a number of challenges because of the complexities of working with an oily substance and the rapid deterioration of immune cell viability before analysis can be performed. Here we present a highly reproducible flow cytometry protocol for the quantification of immune cells in human adipose tissue, which overcomes these issues.

PMID: 32926866 [PubMed - as supplied by publisher]