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Long-term effects of PM2·5 on neurological disorders in the American Medicare population: a longitudinal cohort study.

Author(s): Shi L, Wu X, Danesh Yazdi M, Braun D, Abu Awad Y, Wei Y, Liu P, Di Q, Wang Y, Schwartz J, Dominici F, Kioumourtzoglou MA, Zanobetti A...

BACKGROUND: Accumulating evidence links fine particulate matter (PM2·5) to premature mortality, cardiovascular disease, and respiratory disease. However, less is known about the influence of PM2·5 ...

Article GUID: 33091388
Dynamic modeling of dielectric elastomer actuator with conical shape.

Author(s): Huang P, Ye W, Wang Y

PLoS One. 2020;15(8):e0235229 Authors: Huang P, Ye W, Wang Y

Article GUID: 32797117
Change in PM2.5 exposure and mortality among Medicare recipients: Combining a semi-randomized approach and inverse probability weights in a low exposure population.

Author(s): Awad YA, Di Q, Wang Y, Choirat C, Coull BA, Zanobetti A, Schwartz J

Environ Epidemiol. 2019 Aug;3(4):e054 Authors: Awad YA, Di Q, Wang Y, Choirat C, Coull BA, Zanobetti A, Schwartz J

Article GUID: 31538135
Saccharification efficiencies of multi-enzyme complexes produced by aerobic fungi.

Author(s): Badhan A, Huang J, Wang Y, Abbott DW, Di Falco M, Tsang A, McAllister T

N Biotechnol. 2018 Nov 25;46:1-6 Authors: Badhan A, Huang J, Wang Y, Abbott DW, Di Falco M, Tsang A, McAllister T

Article GUID: 29803771
New recombinant fibrolytic enzymes for improved in vitro ruminal fiber degradability of barley straw.

Author(s): Ribeiro GO, Badhan A, Huang J, Beauchemin KA, Yang W, Wang Y, Tsang A, McAllister TA

J Anim Sci. 2018 Jul 20;: Authors: Ribeiro GO, Badhan A, Huang J, Beauchemin KA, Yang W, Wang Y, Tsang A, McAllister TA

Article GUID: 30053012
Simulating micro-scale thermal interactions in different building environments for mitigating urban heat islands.

Author(s): Chatterjee S, Khan A, Dinda A, Mithun S, Khatun R, Akbari H, Kusaka H, Mitra C, Bhatti SS, Doan QV, Wang Y

Sci Total Environ. 2019 May 01;663:610-631 Authors: Chatterjee S, Khan A, Dinda A, Mithun S, Khatun R, Akbari H, Kusaka H, Mitra C, Bhatti SS, Doan QV, Wang Y

Article GUID: 30731408
Title:New recombinant fibrolytic enzymes for improved in vitro ruminal fiber degradability of barley straw.
Authors:Ribeiro GO,  Badhan A,  Huang J,  Beauchemin KA,  Yang W,  Wang Y,  Tsang A,  McAllister TA
Link:https://www.ncbi.nlm.nih.gov/pubmed/30053012?dopt=Abstract
DOI:10.1093/jas/sky251
Category:J Anim Sci
PMID:30053012
Dept Affiliation: GENOMICS
1 Department of Production Animal Health, Faculty of Veterinary Medicine, University of Calgary, Calgary, AB, Canada.
2 Lethbridge Research and Development Centre, Agriculture and Agri-Food Canada, Lethbridge, AB, Canada.
3 Institute of Biological Resources, Jiangxi Academy of Sciences, Nanchang, China.
4 Centre for Structural and Functional Genomics, Concordia University, Montreal, QC, Canada.

Description:

New recombinant fibrolytic enzymes for improved in vitro ruminal fiber degradability of barley straw.

J Anim Sci. 2018 Jul 20;:

Authors: Ribeiro GO, Badhan A, Huang J, Beauchemin KA, Yang W, Wang Y, Tsang A, McAllister TA

Abstract

This study used a high-throughput in vitro microassay, in vitro batch culture, and the Rumen Simulation Technique (RUSITEC) to screen recombinant fibrolytic enzymes for their ability to increase the ruminal fiber degradability of barley straw. Eleven different recombinant enzymes in combination with a crude mixture of rumen enzymes (50% recombinant enzyme:50% crude mixture of rumen enzymes) were compared with the crude mixture of rumen enzymes alone. In the microassay, all treatments were applied at 15 mg of protein load per gram barley straw glucan. Based on the microassay results, 1 recombinant endoglucanase [EGL7A, from the glycoside hydrolase (GH) family 7], 2 recombinant xylanases (XYL10A and XYL10C, from GH10), and a recombinant enzyme mixture were selected and compared with a crude mixture of fibrolytic enzymes from Aspergillus aculeatus for their ability to hydrolyze barley straw. For batch culture, enzymes were applied to barley straw at 2 dosages (100 and 500 µg of protein/g of substrate DM). All enzymes increased (P < 0.05) DM disappearance and total VFA production, but the mixture of recombinant enzymes was not superior to the use of a single recombinant enzyme. Based on positive results (P < 0.05) for total DM disappearance and VFA production in batch culture, 3 enzymes (EGL7A, XYL10A, and XYL10C) were selected and applied to barley straw at 500 µg of protein per gram for further assessment in RUSITECs fed a concentrate:barley straw diet (300:700 g/kg DM). In RUSITECs, the recombinant enzyme XYL10A increased (P < 0.05) barley straw DM, NDF, and ADF disappearance, whereas EGL7A and XYL10C had no effect. The enzymes selected based on the high-throughput in vitro microassay consistently increased barley straw degradation in ruminal batch culture, but not in the semicontinuous culture RUSITEC system.

PMID: 30053012 [PubMed - as supplied by publisher]