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Long-term effects of PM2·5 on neurological disorders in the American Medicare population: a longitudinal cohort study.

Author(s): Shi L, Wu X, Danesh Yazdi M, Braun D, Abu Awad Y, Wei Y, Liu P, Di Q, Wang Y, Schwartz J, Dominici F, Kioumourtzoglou MA, Zanobetti A...

BACKGROUND: Accumulating evidence links fine particulate matter (PM2·5) to premature mortality, cardiovascular disease, and respiratory disease. However, less is known about the influence of PM2·5 ...

Article GUID: 33091388
Dynamic modeling of dielectric elastomer actuator with conical shape.

Author(s): Huang P, Ye W, Wang Y

PLoS One. 2020;15(8):e0235229 Authors: Huang P, Ye W, Wang Y

Article GUID: 32797117
Change in PM2.5 exposure and mortality among Medicare recipients: Combining a semi-randomized approach and inverse probability weights in a low exposure population.

Author(s): Awad YA, Di Q, Wang Y, Choirat C, Coull BA, Zanobetti A, Schwartz J

Environ Epidemiol. 2019 Aug;3(4):e054 Authors: Awad YA, Di Q, Wang Y, Choirat C, Coull BA, Zanobetti A, Schwartz J

Article GUID: 31538135
Saccharification efficiencies of multi-enzyme complexes produced by aerobic fungi.

Author(s): Badhan A, Huang J, Wang Y, Abbott DW, Di Falco M, Tsang A, McAllister T

N Biotechnol. 2018 Nov 25;46:1-6 Authors: Badhan A, Huang J, Wang Y, Abbott DW, Di Falco M, Tsang A, McAllister T

Article GUID: 29803771
New recombinant fibrolytic enzymes for improved in vitro ruminal fiber degradability of barley straw.

Author(s): Ribeiro GO, Badhan A, Huang J, Beauchemin KA, Yang W, Wang Y, Tsang A, McAllister TA

J Anim Sci. 2018 Jul 20;: Authors: Ribeiro GO, Badhan A, Huang J, Beauchemin KA, Yang W, Wang Y, Tsang A, McAllister TA

Article GUID: 30053012
Simulating micro-scale thermal interactions in different building environments for mitigating urban heat islands.

Author(s): Chatterjee S, Khan A, Dinda A, Mithun S, Khatun R, Akbari H, Kusaka H, Mitra C, Bhatti SS, Doan QV, Wang Y

Sci Total Environ. 2019 May 01;663:610-631 Authors: Chatterjee S, Khan A, Dinda A, Mithun S, Khatun R, Akbari H, Kusaka H, Mitra C, Bhatti SS, Doan QV, Wang Y

Article GUID: 30731408
Title:Saccharification efficiencies of multi-enzyme complexes produced by aerobic fungi.
Authors:Badhan A,  Huang J,  Wang Y,  Abbott DW,  Di Falco M,  Tsang A,  McAllister T
Link:https://www.ncbi.nlm.nih.gov/pubmed/29803771?dopt=Abstract
DOI:10.1016/j.nbt.2018.05.003
Category:N Biotechnol
PMID:29803771
Dept Affiliation: GENOMICS
1 Agriculture and Agri-Food Canada, Lethbridge Research Centre, Lethbridge, Alberta, Canada.
2 Institute of Biological Resources, Jiangxi Academy of Sciences, Nanchang, 330096, China.
3 Centre for Structural and Functional Genomics, Concordia University, Montreal, Quebec, H4B 1R6, Canada.
4 Agriculture and Agri-Food Canada, Lethbridge Research Centre, Lethbridge, Alberta, Canada. Electronic address: tim.mcallister@agr.gc.ca.

Description:

Saccharification efficiencies of multi-enzyme complexes produced by aerobic fungi.

N Biotechnol. 2018 Nov 25;46:1-6

Authors: Badhan A, Huang J, Wang Y, Abbott DW, Di Falco M, Tsang A, McAllister T

Abstract

In the present study, we have characterized high molecular weight multi-enzyme complexes in two commercial enzymes produced by Trichoderma reesei (Spezyme CP) and Penicillium funiculosum (Accellerase XC). We successfully identified 146-1000?kDa complexes using Blue native polyacrylamide gel electrophoresis (BN-PAGE) to fractionate the protein profile in both preparations. Identified complexes dissociated into lower molecular weight constituents when loaded on SDS PAGE. Unfolding of the secondary structure of multi-enzyme complexes with trimethylamine (pH >10) suggested that they were not a result of unspecific protein aggregation. Cellulase (CMCase) profiles of extracts of BN-PAGE fractionated protein bands confirmed cellulase activity within the multi-enzyme complexes. A microassay was used to identify protein bands that promoted high levels of glucose release from barley straw. Those with high saccharification yield were subjected to LC-MS analysis to identify the principal enzymatic activities responsible. The results suggest that secretion of proteins by aerobic fungi leads to the formation of high molecular weight multi-enzyme complexes that display activity against carboxymethyl cellulose and barley straw.

PMID: 29803771 [PubMed - indexed for MEDLINE]