Keyword search (3,448 papers available)


Real-Time Optogenetics System for Controlling Gene Expression Using a Model-Based Design.

Author(s): Soffer G, Perry JM, Shih SCC

Optimization of engineered biological systems requires precise control over the rates and timing of gene expression. Optogenetics is used to dynamically control gene expression as an alternative to conventional chemical-based methods since it provides a mor...

Article GUID: 33543619
Increased oxidative modifications of amniotic fluid albumin in pregnancies associated with gestational diabetes mellitus.

Author(s): Boisvert MR, Koski KG, Skinner CD

Anal Chem. 2010 Feb 01;82(3):1133-7 Authors: Boisvert MR, Koski KG, Skinner CD

Article GUID: 20063865
Development of a particle-trap preconcentration-soft ionization mass spectrometric technique for the quantification of mercury halides in air

Author(s): Deeds DA; Ghoshdastidar A; Raofie F; Guérette ÉA; Tessier A; Ariya PA;

Measurement of oxidized mercury, Hg(II), in the atmosphere poses a significant analytical challenge as Hg(II) is present at ultra-trace concentrations (picograms per cubic meter air). Current technologies are sufficiently sensitive to measure the total Hg p...

Article GUID: 25837315
Integration of World-to-Chip Interfaces with Digital Microfluidics for Bacterial Transformation and Enzymatic Assays.

Author(s): Moazami E, Perry JM, Soffer G, Husser MC, Shih SCC

Anal Chem. 2019 Apr 16;91(8):5159-5168 Authors: Moazami E, Perry JM, Soffer G, Husser MC, Shih SCC

Article GUID: 30945840
Analyzing the Biochemical Alteration of Green Algae During Chronic Exposure to Triclosan Based on Synchrotron-Based Fourier Transform Infrared Spectromicroscopy.

Author(s): Xin X, Huang G, An C, Weger H, Cheng G, Shen J, Rosendahl S

Anal Chem. 2019 May 31;: Authors: Xin X, Huang G, An C, Weger H, Cheng G, Shen J, Rosendahl S

Article GUID: 31117408
Title:Integration of World-to-Chip Interfaces with Digital Microfluidics for Bacterial Transformation and Enzymatic Assays.
Authors:Moazami E,  Perry JM,  Soffer G,  Husser MC,  Shih SCC
Link:https://www.ncbi.nlm.nih.gov/pubmed/30945840?dopt=Abstract
Category:Anal Chem
PMID:30945840
Dept Affiliation: ENCS
1 Department of Electrical and Computer Engineering , Concordia University , Montréal , Québec H3G1M8 , Canada.
2 Centre for Applied Synthetic Biology , Concordia University , Montréal , Québec H4B1R6 , Canada.
3 Department of Biology , Concordia University , Montréal , Québec H4B1R6 , Canada.

Description:

Integration of World-to-Chip Interfaces with Digital Microfluidics for Bacterial Transformation and Enzymatic Assays.

Anal Chem. 2019 Apr 16;91(8):5159-5168

Authors: Moazami E, Perry JM, Soffer G, Husser MC, Shih SCC

Abstract

Digital microfluidics (DMF) represents an alternative to the conventional microfluidic paradigm of transporting fluids in enclosed channels. One of the major benefits of DMF is that fluid motion and control is achieved without external pumps. The automation component of DMF have pushed the barriers of this "lab-on-chip" technology. However, integration with external components (i.e., "world-to-chip") interfaces have been a challenge. Two common "world-to-chip" challenges are (1) delivering biological samples to DMF devices and (2) accurately controlling temperatures on device. To address these challenges, this work describes two "world-to-chip" interface features that have been integrated on a DMF platform: a reagent delivery system and a thermal control apparatus. This platform enables a variety of biological or chemical experiments to be conducted on-chip while reducing manual intervention. Specifically, our platform increases reagent volumes available to device reservoirs volume by at least 50-fold eliminating the need to manually refill reservoirs while improving droplet dispensing reproducibility. In addition, we have integrated a closed-loop temperature control system that offers precise temperature control on-chip. To validate our "world-to-chip" interface, we have automated bacterial transformation and enzymatic assay protocols, showing that such a system enhances DMF performance. Overall, we propose that this system will improve biological experimentation which requires fluidic and temperature control integrated on DMF platforms.

PMID: 30945840 [PubMed - in process]