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Author(s): Dilokpimol A, Peng M, Di Falco M, Chin A Woeng T, Hegi RMW, Granchi Z, Tsang A, Hildén KS, Mäkelä MR, de Vries RP
Bioresour Technol. 2020 May 05;311:123477 Authors: Dilokpimol A, Peng M, Di Falco M, Chin A Woeng T, Hegi RMW, Granchi Z, Tsang A, Hildén KS, Mäkelä MR, de Vries RP
Article GUID: 32408196
Author(s): Guo H, Chen Z, Lu C, Guo J, Li H, Song Y, Han Y, Hou Y
Bioresour Technol. 2020 Feb 22;305:123073 Authors: Guo H, Chen Z, Lu C, Guo J, Li H, Song Y, Han Y, Hou Y
Article GUID: 32145698
Author(s): Li Y, Guo J, Li H, Song Y, Chen Z, Lu C, Han Y, Hou Y
Bioresour Technol. 2019 Oct 28;296:122340 Authors: Li Y, Guo J, Li H, Song Y, Chen Z, Lu C, Han Y, Hou Y
Article GUID: 31704601
Author(s): Guo H, Chen Z, Guo J, Lu C, Song Y, Han Y, Li H, Hou Y
Bioresour Technol. 2019 Jul 16;291:121816 Authors: Guo H, Chen Z, Guo J, Lu C, Song Y, Han Y, Li H, Hou Y
Article GUID: 31344631
Author(s): Mahajan C, Basotra N, Singh S, Di Falco M, Tsang A, Chadha BS
Bioresour Technol. 2016 Jan;200:55-63 Authors: Mahajan C, Basotra N, Singh S, Di Falco M, Tsang A, Chadha BS
Article GUID: 26476165
Author(s): Rai R, Kaur B, Singh S, Di Falco M, Tsang A, Chadha BS
Bioresour Technol. 2016 Sep;216:958-67 Authors: Rai R, Kaur B, Singh S, Di Falco M, Tsang A, Chadha BS
Article GUID: 27341464
Author(s): Neha Basotra
Mycothermus thermophilus (Syn. Scytalidium thermophilum/Humicola insolens), a thermophilic fungus, is being reported to produce appreciable titers of cellulases and hemicellulases during shake flask culturing on cellulose/wheat-bran/rice straw based product...
Article GUID: 27744242
Author(s): Badhan A; Ribeiro GO; Jones DR; Wang Y; Abbott DW; Di Falco M; Tsang A; McAllister TA;
Crude enzyme extracts typically contain a broad spectrum of enzyme activities, most of which are redundant to those naturally produced by the rumen microbiome. Identification of enzyme activities that are synergistic to those produced by the rumen microbiom...
Article GUID: 29621684
Author(s): Chadha BS, Kaur B, Basotra N, Tsang A, Pandey A
Bioresour Technol. 2019 Apr;277:195-203 Authors: Chadha BS, Kaur B, Basotra N, Tsang A, Pandey A
Article GUID: 30679061
Author(s): Yin P, Guo J, Xiao S, Chen Z, Song Y, Ren X
Bioresour Technol. 2019 Mar;276:260-268 Authors: Yin P, Guo J, Xiao S, Chen Z, Song Y, Ren X
Article GUID: 30640020
Author(s): Li K, Guo J, Li H, Han Y, Chen Z, Song Y, Xing Y, Zhang C
Bioresour Technol. 2019 May;279:297-306 Authors: Li K, Guo J, Li H, Han Y, Chen Z, Song Y, Xing Y, Zhang C
Article GUID: 30738356
Title: | Identification of novel enzymes to enhance the ruminal digestion of barley straw |
Authors: | Badhan A, Ribeiro GO, Jones DR, Wang Y, Abbott DW, Di Falco M, Tsang A, McAllister TA, |
Link: | https://pubmed.ncbi.nlm.nih.gov/29621684/ |
DOI: | 10.1016/j.biortech.2018.03.086 |
Category: | Bioresour Technol |
PMID: | 29621684 |
Dept Affiliation: | GENOMICS
1 Agriculture and Agri-Food Canada, Lethbridge Research Centre, Lethbridge, Alberta T1J 4P4, Canada. 2 Centre for Structural and Functional Genomics, Concordia University, Montreal, Quebec H4B 1R6, Canada. 3 Agriculture and Agri-Food Canada, Lethbridge Research Centre, Lethbridge, Alberta T1J 4P4, Canada. Electronic address: tim.mcallister@agr.gc.ca. |
Description: |
Crude enzyme extracts typically contain a broad spectrum of enzyme activities, most of which are redundant to those naturally produced by the rumen microbiome. Identification of enzyme activities that are synergistic to those produced by the rumen microbiome could enable formulation of enzyme cocktails that improve fiber digestion in ruminants. Compared to untreated barley straw, Viscozyme® increased gas production, dry matter digestion (P < 0.01) and volatile fatty acid production (P < 0.001) in ruminal batch cultures. Fractionation of Viscozyme® by Blue Native PAGE and analyses using a microassay and mass-spectrometry revealed a GH74 endoglucanase, GH71 a-1,3-glucanase, GH5 mannanase, GH7 cellobiohydrolase, GH28 pectinase, and esterases from Viscozyme® contributed to enhanced saccharification of barley straw by rumen mix enzymes. Grouping of these identified activities with their carbohydrate active enzymes (CAZy) counterparts enabled selection of similar CAZymes for downstream production and screening. Mining of these specific activities from other biological systems could lead to high value enzyme formulations for ruminants. |