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Sublethal Paraquat Confers Multidrug Tolerance in Pseudomonas aeruginosa by Inducing Superoxide Dismutase Activity and Lowering Envelope Permeability.

Author(s): Martins D, McKay GA, English AM, Nguyen D

Stressors and environmental cues shape the physiological state of bacteria, and thus how they subsequently respond to antibiotic toxicity. To understand how superoxide stress can modulate survival to bactericidal antibiotics, we examined the effect of intra...

Article GUID: 33101252
Ctt1 catalase activity potentiates antifungal azoles in the emerging opportunistic pathogen Saccharomyces cerevisiae.

Author(s): Martins D, Nguyen D, English AM

Sci Rep. 2019 Jun 24;9(1):9185 Authors: Martins D, Nguyen D, English AM

Article GUID: 31235707
Derivatization of yeast cytochrome c peroxidase with pentaammineruthenium(III).

Author(s): Fox T, English AM, Gibbs BF

Derivatization of yeast cytochrome c peroxidase with pentaammineruthenium(III).
Bioconjug Chem. 1994 Jan-Feb;5(1):14-20
Authors: Fox T, English AM, Gibbs BF
Abstract
Cytochrome c peroxidase (CCP) was derivatized using aquopentaamminerutheniu...

Article GUID: 8199229
Mass spectral analysis of protein-based radicals using DBNBS. Nonradical adduct formation versus spin trapping.

Author(s): Filosa A, English AM

J Biol Chem. 2001 Jun 15;276(24):21022-7 Authors: Filosa A, English AM

Article GUID: 11262405
Heme nitrosylation of deoxyhemoglobin by s-nitrosoglutathione requires copper.

Author(s): Romeo AA, Capobianco JA, English AM

J Biol Chem. 2002 Jul 05;277(27):24135-41 Authors: Romeo AA, Capobianco JA, English AM

Article GUID: 11970954
S-nitrosation of Ca(2+)-loaded and Ca(2+)-free recombinant calbindin D(28K) from human brain.

Author(s): Tao L, Murphy ME, English AM

Biochemistry. 2002 May 14;41(19):6185-92 Authors: Tao L, Murphy ME, English AM

Article GUID: 11994015
Different pathways of radical translocation in yeast cytochrome c peroxidase and its W191F mutant on reaction with H(2)O(2) suggest an antioxidant role.

Author(s): Tsaprailis G, English AM

J Biol Inorg Chem. 2003 Feb;8(3):248-55 Authors: Tsaprailis G, English AM

Article GUID: 12589560
Mechanism of S-nitrosation of recombinant human brain calbindin D28K.

Author(s): Tao L, English AM

Biochemistry. 2003 Mar 25;42(11):3326-34 Authors: Tao L, English AM

Article GUID: 12641465
Quantitative analysis of the yeast proteome by incorporation of isotopically labeled leucine.

Author(s): Jiang H, English AM

J Proteome Res. 2002 Jul-Aug;1(4):345-50 Authors: Jiang H, English AM

Article GUID: 12645890
Scavenging with TEMPO* to identify peptide- and protein-based radicals by mass spectrometry: advantages of spin scavenging over spin trapping.

Author(s): Wright PJ, English AM

J Am Chem Soc. 2003 Jul 16;125(28):8655-65 Authors: Wright PJ, English AM

Article GUID: 12848573
ESI-MS and FTIR studies of the interaction between the second PDZ domain of hPTP1E and target peptides.

Author(s): Papp R, Ekiel I, English AM

Biochem Cell Biol. 2003 Apr;81(2):71-80 Authors: Papp R, Ekiel I, English AM

Article GUID: 12870871
Superoxide dismutase targets NO from GSNO to Cysbeta93 of oxyhemoglobin in concentrated but not dilute solutions of the protein.

Author(s): Romeo AA, Capobianco JA, English AM

J Am Chem Soc. 2003 Nov 26;125(47):14370-8 Authors: Romeo AA, Capobianco JA, English AM

Article GUID: 14624585
Protein S-glutathiolation triggered by decomposed S-nitrosoglutathione.

Author(s): Tao L, English AM

Biochemistry. 2004 Apr 06;43(13):4028-38 Authors: Tao L, English AM

Article GUID: 15049710
Mass spectrometric analysis of nitroxyl-mediated protein modification: comparison of products formed with free and protein-based cysteines.

Author(s): Shen B, English AM

Biochemistry. 2005 Oct 25;44(42):14030-44 Authors: Shen B, English AM

Article GUID: 16229492
Hemoglobin S-nitrosation on oxygenation of nitrite/deoxyhemoglobin incubations is attenuated by methemoglobin.

Author(s): Laterreur J, English AM

J Inorg Biochem. 2007 Nov;101(11-12):1827-35 Authors: Laterreur J, English AM

Article GUID: 17889368
ESI-MS quantitation of iron as its 4-(2-pyridylazo)resorcinol (PAR) complex: application to pharmaceutical tablets containing iron oxide pigment.

Author(s): Susanto D, English AM, Sharma R, Kwong E

J Mass Spectrom. 2011 May;46(5):508-16 Authors: Susanto D, English AM, Sharma R, Kwong E

Article GUID: 21520348
SOD1 oxidation and formation of soluble aggregates in yeast: relevance to sporadic ALS development.

Author(s): Martins D, English AM

Redox Biol. 2014;2:632-9 Authors: Martins D, English AM

Article GUID: 24936435
Targeted proteomics identify metabolism-dependent interactors of yeast cytochrome c peroxidase: implications in stress response and heme trafficking.

Author(s): Kathiresan M, English AM

Metallomics. 2016 Apr;8(4):434-43 Authors: Kathiresan M, English AM

Article GUID: 26980054
LC-MS/MS suggests that hole hopping in cytochrome c peroxidase protects its heme from oxidative modification by excess H2O2.

Author(s): Kathiresan M, English AM

Chem Sci. 2017 Feb 01;8(2):1152-1162 Authors: Kathiresan M, English AM

Article GUID: 28451256
Superoxide dismutase activity confers (p)ppGpp-mediated antibiotic tolerance to stationary-phase Pseudomonas aeruginosa.

Author(s): Martins D, McKay G, Sampathkumar G, Khakimova M, English AM, Nguyen D

Proc Natl Acad Sci U S A. 2018 09 25;115(39):9797-9802 Authors: Martins D, McKay G, Sampathkumar G, Khakimova M, English AM, Nguyen D

Article GUID: 30201715
Title:Heme nitrosylation of deoxyhemoglobin by s-nitrosoglutathione requires copper.
Authors:Romeo AA,  Capobianco JA,  English AM
Link:https://www.ncbi.nlm.nih.gov/pubmed/11970954?dopt=Abstract
DOI:10.1074/jbc.M202221200
Category:J Biol Chem
PMID:11970954
Dept Affiliation: CHEMBIOCHEM
1 Department of Chemistry and Biochemistry, Concordia University, 1455 de Maisonneuve Boulevard West, Montreal, Quebec H3G 1M8, Canada.

Description:

Heme nitrosylation of deoxyhemoglobin by s-nitrosoglutathione requires copper.

J Biol Chem. 2002 Jul 05;277(27):24135-41

Authors: Romeo AA, Capobianco JA, English AM

Abstract

NO reactions with hemoglobin (Hb) likely play a role in blood pressure regulation. For example, NO exchange between Hb and S-nitrosoglutathione (GSNO) has been reported in vitro. Here we examine the reaction between GSNO and deoxyHb (HbFe(II)) in the presence of both Cu(I) (2,9-dimethyl-1, 10-phenanthroline (neocuproine)) and Cu(II) (diethylenetriamine-N,N,N',N",N"-pentaacetic acid) chelators using a copper-depleted Hb solution. Spectroscopic analysis of deoxyHb (HbFe(II))/GSNO incubates shows prompt formation (<5 min) of approximately 100% heme-nitrosylated Hb (HbFe(II)NO) in the absence of chelators, 46% in the presence of diethylenetriamine-N,N,N',N",N"-pentaacetic acid, and 25% in the presence of neocuproine. Negligible (<2%) HbFe(II)NO was detected when neocuproine was added to copper-depleted HbFe(II)/GSNO incubates. Thus, HbFe(II)NO formation via a mechanism involving free NO generated by Cu(I) catalysis of GSNO breakdown is proposed. GSH is a source of reducing equivalents because extensive GSSG was detected in HbFe(II)/GSNO incubates in the absence of metal chelators. No S-nitrosation of HbFe(II) was detected under any conditions. In contrast, the NO released from GSNO is directed to Cysbeta(93) of oxyHb in the absence of chelators, but only metHb formation is observed in the presence of chelators. Our findings reveal that the reactions of GSNO and Hb are controlled by copper and that metal chelators do not fully inhibit NO release from GSNO in Hb-containing solutions.

PMID: 11970954 [PubMed - indexed for MEDLINE]