Keyword search (3,448 papers available)


Sublethal Paraquat Confers Multidrug Tolerance in Pseudomonas aeruginosa by Inducing Superoxide Dismutase Activity and Lowering Envelope Permeability.

Author(s): Martins D, McKay GA, English AM, Nguyen D

Stressors and environmental cues shape the physiological state of bacteria, and thus how they subsequently respond to antibiotic toxicity. To understand how superoxide stress can modulate survival to bactericidal antibiotics, we examined the effect of intra...

Article GUID: 33101252
Ctt1 catalase activity potentiates antifungal azoles in the emerging opportunistic pathogen Saccharomyces cerevisiae.

Author(s): Martins D, Nguyen D, English AM

Sci Rep. 2019 Jun 24;9(1):9185 Authors: Martins D, Nguyen D, English AM

Article GUID: 31235707
Derivatization of yeast cytochrome c peroxidase with pentaammineruthenium(III).

Author(s): Fox T, English AM, Gibbs BF

Derivatization of yeast cytochrome c peroxidase with pentaammineruthenium(III).
Bioconjug Chem. 1994 Jan-Feb;5(1):14-20
Authors: Fox T, English AM, Gibbs BF
Abstract
Cytochrome c peroxidase (CCP) was derivatized using aquopentaamminerutheniu...

Article GUID: 8199229
Mass spectral analysis of protein-based radicals using DBNBS. Nonradical adduct formation versus spin trapping.

Author(s): Filosa A, English AM

J Biol Chem. 2001 Jun 15;276(24):21022-7 Authors: Filosa A, English AM

Article GUID: 11262405
Heme nitrosylation of deoxyhemoglobin by s-nitrosoglutathione requires copper.

Author(s): Romeo AA, Capobianco JA, English AM

J Biol Chem. 2002 Jul 05;277(27):24135-41 Authors: Romeo AA, Capobianco JA, English AM

Article GUID: 11970954
S-nitrosation of Ca(2+)-loaded and Ca(2+)-free recombinant calbindin D(28K) from human brain.

Author(s): Tao L, Murphy ME, English AM

Biochemistry. 2002 May 14;41(19):6185-92 Authors: Tao L, Murphy ME, English AM

Article GUID: 11994015
Different pathways of radical translocation in yeast cytochrome c peroxidase and its W191F mutant on reaction with H(2)O(2) suggest an antioxidant role.

Author(s): Tsaprailis G, English AM

J Biol Inorg Chem. 2003 Feb;8(3):248-55 Authors: Tsaprailis G, English AM

Article GUID: 12589560
Mechanism of S-nitrosation of recombinant human brain calbindin D28K.

Author(s): Tao L, English AM

Biochemistry. 2003 Mar 25;42(11):3326-34 Authors: Tao L, English AM

Article GUID: 12641465
Quantitative analysis of the yeast proteome by incorporation of isotopically labeled leucine.

Author(s): Jiang H, English AM

J Proteome Res. 2002 Jul-Aug;1(4):345-50 Authors: Jiang H, English AM

Article GUID: 12645890
Scavenging with TEMPO* to identify peptide- and protein-based radicals by mass spectrometry: advantages of spin scavenging over spin trapping.

Author(s): Wright PJ, English AM

J Am Chem Soc. 2003 Jul 16;125(28):8655-65 Authors: Wright PJ, English AM

Article GUID: 12848573
ESI-MS and FTIR studies of the interaction between the second PDZ domain of hPTP1E and target peptides.

Author(s): Papp R, Ekiel I, English AM

Biochem Cell Biol. 2003 Apr;81(2):71-80 Authors: Papp R, Ekiel I, English AM

Article GUID: 12870871
Superoxide dismutase targets NO from GSNO to Cysbeta93 of oxyhemoglobin in concentrated but not dilute solutions of the protein.

Author(s): Romeo AA, Capobianco JA, English AM

J Am Chem Soc. 2003 Nov 26;125(47):14370-8 Authors: Romeo AA, Capobianco JA, English AM

Article GUID: 14624585
Protein S-glutathiolation triggered by decomposed S-nitrosoglutathione.

Author(s): Tao L, English AM

Biochemistry. 2004 Apr 06;43(13):4028-38 Authors: Tao L, English AM

Article GUID: 15049710
Mass spectrometric analysis of nitroxyl-mediated protein modification: comparison of products formed with free and protein-based cysteines.

Author(s): Shen B, English AM

Biochemistry. 2005 Oct 25;44(42):14030-44 Authors: Shen B, English AM

Article GUID: 16229492
Hemoglobin S-nitrosation on oxygenation of nitrite/deoxyhemoglobin incubations is attenuated by methemoglobin.

Author(s): Laterreur J, English AM

J Inorg Biochem. 2007 Nov;101(11-12):1827-35 Authors: Laterreur J, English AM

Article GUID: 17889368
ESI-MS quantitation of iron as its 4-(2-pyridylazo)resorcinol (PAR) complex: application to pharmaceutical tablets containing iron oxide pigment.

Author(s): Susanto D, English AM, Sharma R, Kwong E

J Mass Spectrom. 2011 May;46(5):508-16 Authors: Susanto D, English AM, Sharma R, Kwong E

Article GUID: 21520348
SOD1 oxidation and formation of soluble aggregates in yeast: relevance to sporadic ALS development.

Author(s): Martins D, English AM

Redox Biol. 2014;2:632-9 Authors: Martins D, English AM

Article GUID: 24936435
Targeted proteomics identify metabolism-dependent interactors of yeast cytochrome c peroxidase: implications in stress response and heme trafficking.

Author(s): Kathiresan M, English AM

Metallomics. 2016 Apr;8(4):434-43 Authors: Kathiresan M, English AM

Article GUID: 26980054
LC-MS/MS suggests that hole hopping in cytochrome c peroxidase protects its heme from oxidative modification by excess H2O2.

Author(s): Kathiresan M, English AM

Chem Sci. 2017 Feb 01;8(2):1152-1162 Authors: Kathiresan M, English AM

Article GUID: 28451256
Superoxide dismutase activity confers (p)ppGpp-mediated antibiotic tolerance to stationary-phase Pseudomonas aeruginosa.

Author(s): Martins D, McKay G, Sampathkumar G, Khakimova M, English AM, Nguyen D

Proc Natl Acad Sci U S A. 2018 09 25;115(39):9797-9802 Authors: Martins D, McKay G, Sampathkumar G, Khakimova M, English AM, Nguyen D

Article GUID: 30201715
Title:Sublethal Paraquat Confers Multidrug Tolerance in Pseudomonas aeruginosa by Inducing Superoxide Dismutase Activity and Lowering Envelope Permeability.
Authors:Martins D,  McKay GA,  English AM,  Nguyen D
Link:https://www.ncbi.nlm.nih.gov/pubmed/33101252
DOI:10.3389/fmicb.2020.576708
Category:Front Microbiol
PMID:33101252
Dept Affiliation: CHEMBIOCHEM
1 Meakins-Christie Laboratories, Research Institute of the McGill University Health Centre, Montreal, QC, Canada.
2 Department of Microbiology and Immunology, McGill University, Montreal, QC, Canada.
3 Department of Chemistry and Biochemistry, Concordia University, Montreal, QC, Canada.
4 Department of Medicine, McGill University, Montreal, QC, Canada.

Description:

Sublethal Paraquat Confers Multidrug Tolerance in Pseudomonas aeruginosa by Inducing Superoxide Dismutase Activity and Lowering Envelope Permeability.

Front Microbiol. 2020; 11:576708

Authors: Martins D, McKay GA, English AM, Nguyen D

Abstract

Stressors and environmental cues shape the physiological state of bacteria, and thus how they subsequently respond to antibiotic toxicity. To understand how superoxide stress can modulate survival to bactericidal antibiotics, we examined the effect of intracellular superoxide generators, paraquat and menadione, on stationary-phase antibiotic tolerance of the opportunistic pathogen, Pseudomonas aeruginosa. We tested how pre-challenge with sublethal paraquat and menadione alters the tolerance to ofloxacin and meropenem in wild-type P. aeruginosa and mutants lacking superoxide dismutase (SOD) activity (sodAB), the paraquat responsive regulator soxR, (p)ppGpp signaling (relA spoT mutant), or the alternative sigma factor rpoS. We confirmed that loss of SOD activity impairs ofloxacin and meropenem tolerance in stationary phase cells, and found that sublethal superoxide generators induce drug tolerance by stimulating SOD activity. This response is rapid, requires de novo protein synthesis, and is RpoS-dependent but does not require (p)ppGpp signaling nor SoxR. We further showed that pre-challenge with sublethal paraquat induces a SOD-dependent reduction in cell-envelope permeability and ofloxacin penetration. Our results highlight a novel mechanism of hormetic protection by superoxide generators, which may have important implications for stress-induced antibiotic tolerance in P. aeruginosa cells.

PMID: 33101252 [PubMed]