Keyword search (3,448 papers available)


Global view of the Clostridium thermocellum cellulosome revealed by quantitative proteomic analysis.

Author(s): Gold ND, Martin VJ

J Bacteriol. 2007 Oct;189(19):6787-95 Authors: Gold ND, Martin VJ

Article GUID: 17644599
Proteomic analysis of Clostridium thermocellum ATCC 27405 reveals the upregulation of an alternative transhydrogenase-malate pathway and nitrogen assimilation in cells grown on cellulose.

Author(s): Burton E, Martin VJ

Can J Microbiol. 2012 Dec;58(12):1378-88 Authors: Burton E, Martin VJ

Article GUID: 23210995
Expression of a library of fungal β-glucosidases in Saccharomyces cerevisiae for the development of a biomass fermenting strain.

Author(s): Wilde C, Gold ND, Bawa N, Tambor JH, Mougharbel L, Storms R, Martin VJ

Appl Microbiol Biotechnol. 2012 Aug;95(3):647-59 Authors: Wilde C, Gold ND, Bawa N, Tambor JH, Mougharbel L, Storms R, Martin VJ

Article GUID: 22218767
Effects of synthetic cohesin-containing scaffold protein architecture on binding dockerin-enzyme fusions on the surface of Lactococcus lactis.

Author(s): Wieczorek AS, Martin VJ

Microb Cell Fact. 2012 Dec 15;11:160 Authors: Wieczorek AS, Martin VJ

Article GUID: 23241215
Reconstitution of a 10-gene pathway for synthesis of the plant alkaloid dihydrosanguinarine in Saccharomyces cerevisiae.

Author(s): Fossati E, Ekins A, Narcross L, Zhu Y, Falgueyret JP, Beaudoin GA, Facchini PJ, Martin VJ

Nat Commun. 2014;5:3283 Authors: Fossati E, Ekins A, Narcross L, Zhu Y, Falgueyret JP, Beaudoin GA, Facchini PJ, Martin VJ

Article GUID: 24513861
Deconstructing the genetic basis of spent sulphite liquor tolerance using deep sequencing of genome-shuffled yeast.

Author(s): Pinel D, Colatriano D, Jiang H, Lee H, Martin VJ

Biotechnol Biofuels. 2015;8:53 Authors: Pinel D, Colatriano D, Jiang H, Lee H, Martin VJ

Article GUID: 25866561
Synthesis of Morphinan Alkaloids in Saccharomyces cerevisiae.

Author(s): Fossati E, Narcross L, Ekins A, Falgueyret JP, Martin VJ

PLoS One. 2015;10(4):e0124459 Authors: Fossati E, Narcross L, Ekins A, Falgueyret JP, Martin VJ

Article GUID: 25905794
An enzyme-coupled biosensor enables (S)-reticuline production in yeast from glucose.

Author(s): DeLoache WC, Russ ZN, Narcross L, Gonzales AM, Martin VJ, Dueber JE

Nat Chem Biol. 2015 Jul;11(7):465-71 Authors: DeLoache WC, Russ ZN, Narcross L, Gonzales AM, Martin VJ, Dueber JE

Article GUID: 25984720
Metabolic engineering of a tyrosine-overproducing yeast platform using targeted metabolomics.

Author(s): Gold ND, Gowen CM, Lussier FX, Cautha SC, Mahadevan R, Martin VJ

Microb Cell Fact. 2015 May 28;14:73 Authors: Gold ND, Gowen CM, Lussier FX, Cautha SC, Mahadevan R, Martin VJ

Article GUID: 26016674
Directed evolution of a fungal β-glucosidase in Saccharomyces cerevisiae.

Author(s): Larue K, Melgar M, Martin VJ

Biotechnol Biofuels. 2016;9:52 Authors: Larue K, Melgar M, Martin VJ

Article GUID: 26949413
Engineering of a Nepetalactol-Producing Platform Strain of Saccharomyces cerevisiae for the Production of Plant Seco-Iridoids.

Author(s): Campbell A, Bauchart P, Gold ND, Zhu Y, De Luca V, Martin VJ

ACS Synth Biol. 2016 05 20;5(5):405-14 Authors: Campbell A, Bauchart P, Gold ND, Zhu Y, De Luca V, Martin VJ

Article GUID: 26981892
Seamless site-directed mutagenesis of the Saccharomyces cerevisiae genome using CRISPR-Cas9.

Author(s): Biot-Pelletier D, Martin VJ

J Biol Eng. 2016;10:6 Authors: Biot-Pelletier D, Martin VJ

Article GUID: 27134651
Reconstituting Plant Secondary Metabolism in Saccharomyces cerevisiae for Production of High-Value Benzylisoquinoline Alkaloids.

Author(s): Pyne ME, Narcross L, Fossati E, Bourgeois L, Burton E, Gold ND, Martin VJ

Methods Enzymol. 2016;575:195-224 Authors: Pyne ME, Narcross L, Fossati E, Bourgeois L, Burton E, Gold ND, Martin VJ

Article GUID: 27417930
Mining Enzyme Diversity of Transcriptome Libraries through DNA Synthesis for Benzylisoquinoline Alkaloid Pathway Optimization in Yeast.

Author(s): Narcross L, Bourgeois L, Fossati E, Burton E, Martin VJ

ACS Synth Biol. 2016 12 16;5(12):1505-1518 Authors: Narcross L, Bourgeois L, Fossati E, Burton E, Martin VJ

Article GUID: 27442619
Persistence of Escherichia coli in batch and continuous vermicomposting systems.

Author(s): Hénault-Ethier L, Martin VJ, Gélinas Y

Waste Manag. 2016 Oct;56:88-99 Authors: Hénault-Ethier L, Martin VJ, Gélinas Y

Article GUID: 27499290
Title:Deconstructing the genetic basis of spent sulphite liquor tolerance using deep sequencing of genome-shuffled yeast.
Authors:Pinel D,  Colatriano D,  Jiang H,  Lee H,  Martin VJ
Link:https://www.ncbi.nlm.nih.gov/pubmed/25866561?dopt=Abstract
DOI:10.1186/s13068-015-0241-z
Category:Biotechnol Biofuels
PMID:25866561
Dept Affiliation: GENOMICS
1 Department of Biology, Centre for Structural and Functional Genomics, Concordia University, 7141 Sherbrooke Street West, Montréal, Québec H4B 1R6 Canada ; Current address: Energy Biosciences Institute, University of California, Berkeley, Berkeley, CA 94704 USA.
2 Department of Biology, Centre for Structural and Functional Genomics, Concordia University, 7141 Sherbrooke Street West, Montréal, Québec H4B 1R6 Canada.
3 Department of Biology, Centre for Structural and Functional Genomics, Concordia University, 7141 Sherbrooke Street West, Montréal, Québec H4B 1R6 Canada ; Current address: Crabtree Nutrition Laboratories, McGill University Health Center, Montreal, Quebec H3A 1A1 Canada.
4 School of Environmental Sciences, University of Guelph, Guelph, Ontario N1G 2 W1 Canada.

Description:

Deconstructing the genetic basis of spent sulphite liquor tolerance using deep sequencing of genome-shuffled yeast.

Biotechnol Biofuels. 2015;8:53

Authors: Pinel D, Colatriano D, Jiang H, Lee H, Martin VJ

Abstract

BACKGROUND: Identifying the genetic basis of complex microbial phenotypes is currently a major barrier to our understanding of multigenic traits and our ability to rationally design biocatalysts with highly specific attributes for the biotechnology industry. Here, we demonstrate that strain evolution by meiotic recombination-based genome shuffling coupled with deep sequencing can be used to deconstruct complex phenotypes and explore the nature of multigenic traits, while providing concrete targets for strain development.

RESULTS: We determined genomic variations found within Saccharomyces cerevisiae previously evolved in our laboratory by genome shuffling for tolerance to spent sulphite liquor. The representation of these variations was backtracked through parental mutant pools and cross-referenced with RNA-seq gene expression analysis to elucidate the importance of single mutations and key biological processes that play a role in our trait of interest. Our findings pinpoint novel genes and biological determinants of lignocellulosic hydrolysate inhibitor tolerance in yeast. These include the following: protein homeostasis constituents, including Ubp7p and Art5p, related to ubiquitin-mediated proteolysis; stress response transcriptional repressor, Nrg1p; and NADPH-dependent glutamate dehydrogenase, Gdh1p. Reverse engineering a prominent mutation in ubiquitin-specific protease gene UBP7 in a laboratory S. cerevisiae strain effectively increased spent sulphite liquor tolerance.

CONCLUSIONS: This study advances understanding of yeast tolerance mechanisms to inhibitory substrates and biocatalyst design for a biomass-to-biofuel/biochemical industry, while providing insights into the process of mutation accumulation that occurs during genome shuffling.

PMID: 25866561 [PubMed]