Keyword search (3,448 papers available)


Partial purification, kinetic analysis, and amino acid sequence information of a flavonol 3-O-methyltransferase from Serratula tinctoria.

Author(s): Huang TS, Anzellotti D, Dedaldechamp F, Ibrahim RK

Plant Physiol. 2004 Apr;134(4):1366-76 Authors: Huang TS, Anzellotti D, Dedaldechamp F, Ibrahim RK

Article GUID: 15084728
Effects of surfactants on rhizodegradation of oil in a contaminated soil.

Author(s): Memarian R, Ramamurthy AS

J Environ Sci Health A Tox Hazard Subst Environ Eng. 2012;47(10):1486-90 Authors: Memarian R, Ramamurthy AS

Article GUID: 22571537
Enzymatic assay for GHB determination in forensic matrices.

Author(s): Grenier V, Huppé G, Lamarche M, Mireault P

J Anal Toxicol. 2012 Sep;36(7):523-8 Authors: Grenier V, Huppé G, Lamarche M, Mireault P

Article GUID: 22722059
Odorous gaseous emissions as influence by process condition for the forced aeration composting of pig slaughterhouse sludge.

Author(s): Blazy V, de Guardia A, Benoist JC, Daumoin M, Lemasle M, Wolbert D, Barrington S

Waste Manag. 2014 Jul;34(7):1125-38 Authors: Blazy V, de Guardia A, Benoist JC, Daumoin M, Lemasle M, Wolbert D, Barrington S

Article GUID: 24768513
Development of a particle-trap preconcentration-soft ionization mass spectrometric technique for the quantification of mercury halides in air

Author(s): Deeds DA; Ghoshdastidar A; Raofie F; Guérette ÉA; Tessier A; Ariya PA;

Measurement of oxidized mercury, Hg(II), in the atmosphere poses a significant analytical challenge as Hg(II) is present at ultra-trace concentrations (picograms per cubic meter air). Current technologies are sufficiently sensitive to measure the total Hg p...

Article GUID: 25837315
Lithocholic bile acid accumulated in yeast mitochondria orchestrates a development of an anti-aging cellular pattern by causing age-related changes in cellular proteome.

Author(s): Beach A, Richard VR, Bourque S, Boukh-Viner T, Kyryakov P, Gomez-Perez A, Arlia-Ciommo A, Feldman R, Leonov A, Piano A, Svistkova V, Titorenko VI

Cell Cycle. 2015;14(11):1643-56 Authors: Beach A, Richard VR, Bourque S, Boukh-Viner T, Kyryakov P, Gomez-Perez A, Arlia-Ciommo A, Feldman R, Leonov A, Piano A, Svistkova V, Titorenko VI

Article GUID: 25839782
Electrochemical efficacy of a carboxylated multiwalled carbon nanotube filter for the removal of ibuprofen from aqueous solutions under acidic conditions.

Author(s): Bakr AR, Rahaman MS

Chemosphere. 2016 Jun;153:508-20 Authors: Bakr AR, Rahaman MS

Article GUID: 27035389
On-chip integration of droplet microfluidics and nanostructure-initiator mass spectrometry for enzyme screening

Author(s): Joshua Heinemann

Biological assays often require expensive reagents and tedious manipulations. These shortcomings can be overcome using digitally operated microfluidic devices that require reduced sample volumes to automate assays. One particular challenge is integrating bi...

Article GUID: 27957569
Varying the rate of intravenous cocaine infusion influences the temporal dynamics of both drug and dopamine concentrations in the striatum

Author(s): Minogianis EA; Shams WM; Mabrouk OS; Wong JT; Brake WG; Kennedy RT; du Souich P; Samaha AN;...

The faster drugs of abuse reach the brain, the greater is the risk of addiction. Even small differences in the rate of drug delivery can influence outcome. Infusing cocaine intravenously over 5 vs....

Article GUID: 29757478
Title:Enzymatic assay for GHB determination in forensic matrices.
Authors:Grenier V,  Huppé G,  Lamarche M,  Mireault P
Link:https://www.ncbi.nlm.nih.gov/pubmed/22722059?dopt=Abstract
DOI:10.1093/jat/bks053
Category:J Anal Toxicol
PMID:22722059
Dept Affiliation: MASSSPEC
1 Concordia University, Montreal, Quebec, Canada.

Description:

Enzymatic assay for GHB determination in forensic matrices.

J Anal Toxicol. 2012 Sep;36(7):523-8

Authors: Grenier V, Huppé G, Lamarche M, Mireault P

Abstract

Current procedures for the determination of gamma-hydroxybutyric acid (GHB) require time-consuming extraction and derivatization steps before chromatographic detection, making a high-throughput alternative desirable. Bühlmann Laboratories offers an enzymatic assay for the quantitative determination of GHB in urine and serum. We report the adaptation of this photometric assay to the Thermo Scientific MGC-240 analyzer and its use in the determination of GHB in forensic matrices including urine, whole blood and vitreous humour. Most matrices require only a brief centrifugation before analysis, while blood requires an additional protein precipitation step. A variety of cases (sexual assaults, impaired drivers and death investigations) have been analyzed alongside the gas chromatography-mass spectrometry (GC-MS) reference method. Correlation with the GC-MS has been found to be acceptable, with no false negatives and few false positives, although postmortem samples appear more prone to testing false positive than do antemortem samples. Simple sample preparation and high throughput allow for a significant reduction in analysis time relative to chromatographic methods. This assay is used as a screening method in our laboratory, with a quantitative GC-MS method serving for the confirmation of positive results. To our knowledge, this represents the first evaluation of an enzymatic assay for GHB in a forensic context.

PMID: 22722059 [PubMed - indexed for MEDLINE]