Keyword search (3,171 papers available)


Histone H3 lysine 4 trimethylation in sperm is transmitted to the embryo and associated with diet-induced phenotypes in the offspring.

Author(s): Lismer A, Dumeaux V, Lafleur C, Lambrot R, Brind'Amour J, Lorincz MC, Kimmins S

A father's lifestyle impacts offspring health; yet, the underlying molecular mechanisms remain elusive. We hypothesized that a diet that changes methyl donor availability will alter the sperm and embryo epigenomes to impact embryonic gene expression and...

Article GUID: 33596408
Atrx Deletion in Neurons Leads to Sexually Dimorphic Dysregulation of miR-137 and Spatial Learning and Memory Deficits.

Author(s): Tamming RJ, Dumeaux V, Jiang Y, Shafiq S, Langlois L, Ellegood J, Qiu LR, Lerch JP, Bérubé NG

Cell Rep. 2020 Jun 30;31(13):107838 Authors: Tamming RJ, Dumeaux V, Jiang Y, Shafiq S, Langlois L, Ellegood J, Qiu LR, Lerch JP, Bérubé NG

Article GUID: 32610139
Histone deacetylase 1 and 2 drive differentiation and fusion of progenitor cells in human placental trophoblasts.

Author(s): Jaju Bhattad G, Jeyarajah MJ, McGill MG, Dumeaux V, Okae H, Arima T, Lajoie P, Bérubé NG, Renaud SJ

Cell Death Dis. 2020 May 04;11(5):311 Authors: Jaju Bhattad G, Jeyarajah MJ, McGill MG, Dumeaux V, Okae H, Arima T, Lajoie P, Bérubé NG, Renaud SJ

Article GUID: 32366868
The time-varying effect of radiotherapy after breast-conserving surgery for DCIS.

Author(s): Rakovitch E, Sutradhar R, Hallett M, Thompson AM, Gu S, Dumeaux V, Whelan TJ, Paszat L

Breast Cancer Res Treat. 2019 Jul 31;: Authors: Rakovitch E, Sutradhar R, Hallett M, Thompson AM, Gu S, Dumeaux V, Whelan TJ, Paszat L

Article GUID: 31368035
Screening of Candida albicans GRACE library revealed a unique pattern of biofilm formation under repression of the essential gene ILS1.

Author(s): Costa ACBP, Omran RP, Correia-Mesquita TO, Dumeaux V, Whiteway M

Sci Rep. 2019 Jun 24;9(1):9187 Authors: Costa ACBP, Omran RP, Correia-Mesquita TO, Dumeaux V, Whiteway M

Article GUID: 31235750
Interactions between the tumor and the blood systemic response of breast cancer patients.

Author(s): Dumeaux V, Fjukstad B, Fjosne HE, Frantzen JO, Holmen MM, Rodegerdts E, Schlichting E, Børresen-Dale AL, Bongo LA, Lund E, Hallett M

PLoS Comput Biol. 2017 Sep;13(9):e1005680 Authors: Dumeaux V, Fjukstad B, Fjosne HE, Frantzen JO, Holmen MM, Rodegerdts E, Schlichting E, Børresen-Dale AL, Bongo LA, Lund E, Hallett M

Article GUID: 28957325
Mms21: A Putative SUMO E3 Ligase in Candida albicans That Negatively Regulates Invasiveness and Filamentation, and Is Required for the Genotoxic and Cellular Stress Response.

Author(s): Islam A, Tebbji F, Mallick J, Regan H, Dumeaux V, Omran RP, Whiteway M

Genetics. 2019 02;211(2):579-595 Authors: Islam A, Tebbji F, Mallick J, Regan H, Dumeaux V, Omran RP, Whiteway M

Article GUID: 30530734
Role of SUMOylation in differential ERα transcriptional repression by tamoxifen and fulvestrant in breast cancer cells.

Author(s): Traboulsi T, El Ezzy M, Dumeaux V, Audemard E, Mader S

Oncogene. 2019 02;38(7):1019-1037 Authors: Traboulsi T, El Ezzy M, Dumeaux V, Audemard E, Mader S

Article GUID: 30190545
Title:Role of SUMOylation in differential ERα transcriptional repression by tamoxifen and fulvestrant in breast cancer cells.
Authors:Traboulsi T,  El Ezzy M,  Dumeaux V,  Audemard E,  Mader S
Link:https://www.ncbi.nlm.nih.gov/pubmed/30190545?dopt=Abstract
Category:Oncogene
PMID:30190545
Dept Affiliation: PERFORM
1 Institute for Research in Immunology and Cancer, Montréal, QC, H3C 3J7, Canada.
2 Department of Biochemistry and Molecular Medicine, Université de Montréal, Montréal, QC, H3C 3J7, Canada.
3 PERFORM Centre, Concordia University, Montréal, QC, H4B 1R6, Canada.
4 Institute for Research in Immunology and Cancer, Montréal, QC, H3C 3J7, Canada. sylvie.mader@umontreal.ca.
5 Department of Biochemistry and Molecular Medicine, Université de Montréal, Montréal, QC, H3C 3J7, Canada. sylvie.mader@umontreal.ca.

Description:

Role of SUMOylation in differential ERa transcriptional repression by tamoxifen and fulvestrant in breast cancer cells.

Oncogene. 2019 02;38(7):1019-1037

Authors: Traboulsi T, El Ezzy M, Dumeaux V, Audemard E, Mader S

Abstract

Antiestrogens (AEs) are widely used for treatment of estrogen receptor alpha (ERa)-positive breast cancer, but display variable degrees of partial agonism in estrogen target tissues and breast cancer (BC) cells. The fact that BC cells resistant to selective ER modulators (SERMs) like tamoxifen (Tam) can still be sensitive to pure AEs, also called selective ER downregulators, suggests different mechanisms of action, some of which may contribute to the more complete suppression of estrogen target genes by pure AEs. We report herein that pure AEs such as fulvestrant induce transient binding of ERa to DNA, followed by rapid release after 30-40?min without loss of nuclear localization. Loss of DNA binding preceded receptor degradation and was not prevented by proteasome inhibition. Chromatin was less accessible in the presence of fulvestrant than with estradiol or Tam as early as 20?min following treatment, suggesting that chromatin remodeling by pure AEs at ERa target regions prevents transcription in spite of receptor binding. SUMO2/3 marks were detected on chromatin at the peak of ERa binding in cells treated with pure AEs, but not SERMs. Furthermore, decreasing SUMOylation by overexpressing the deSUMOylase SENP1 significantly delayed receptor release from DNA and de-repressed expression of estrogen target genes in the presence of fulvestrant, both in ERa-expressing MCF-7 cells and in transiently transfected ER-negative SK-BR-3 cells. Finally, mutation V534E, identified in a breast metastasis resistant to hormonal therapies, prevented ERa modification and resulted in increased transcriptional activity of estrogen target genes in the presence of fulvestrant in SK-BR-3 cells. Together, our results establish a role for SUMOylation in achieving a more complete transcriptional shut-off of estrogen target genes by pure AEs vs. SERMs in BC cells.

PMID: 30190545 [PubMed - indexed for MEDLINE]