Keyword search (3,619 papers available)


A reliable, reproducible flow cytometry protocol for immune cell quantification in human adipose tissue.

Author(s): Delaney KZ, Dam V, Murphy J, Morais JA, Denis R, Atlas H, Pescarus R, Garneau PY, Santosa S...

The ability to accurately identify and quantify immune cell populations within adipose tissue is important in understanding the role of immune cells in metabolic disease risk. Flow cytometry is the...

Article GUID: 32926866
Acetyl-CoA regulation, OXPHOS integrity and leptin level are different in females with different onsets of obesity.

Author(s): Tam BT, Murphy J, Khor N, Morais JA, Santosa S

Although childhood-onset obesity (CO) and adult-onset obesity (AO) are known to lead to distinctive clinical manifestations and disease risks, the fundamental differences between them are largely unclear. The aim of the current study is to investigate the f...

Article GUID: 32808657
Obesity and ageing: Two sides of the same coin.

Author(s): Tam BT, Morais JA, Santosa S

Obes Rev. 2020 Feb 05;: Authors: Tam BT, Morais JA, Santosa S

Article GUID: 32020741
Acute Testosterone Deficiency Alters Adipose Tissue Fatty Acid Storage.

Author(s): Santosa S, Bush NC, Jensen MD

J Clin Endocrinol Metab. 2017 08 01;102(8):3056-3064 Authors: Santosa S, Bush NC, Jensen MD

Article GUID: 28641384
Intra-Abdominal Adipose Tissue Quantification by Alternative Versus Reference Methods: A Systematic Review and Meta-Analysis.

Author(s): Murphy J, Bacon SL, Morais JA, Tsoukas MA, Santosa S

Obesity (Silver Spring). 2019 May 27;: Authors: Murphy J, Bacon SL, Morais JA, Tsoukas MA, Santosa S

Article GUID: 31131996
Effects of weight loss via high fat vs. low fat alternate day fasting diets on free fatty acid profiles.

Author(s): Varady KA, Dam VT, Klempel MC, Horne M, Cruz R, Kroeger CM, Santosa S

Sci Rep. 2015 Jan 05;5:7561 Authors: Varady KA, Dam VT, Klempel MC, Horne M, Cruz R, Kroeger CM, Santosa S

Article GUID: 25557754
The Sexual Dimorphism of Lipid Kinetics in Humans.

Author(s): Santosa S, Jensen MD

Front Endocrinol (Lausanne). 2015;6:103 Authors: Santosa S, Jensen MD

Article GUID: 26191040
From neutrophils to macrophages: differences in regional adipose tissue depots.

Author(s): Dam V, Sikder T, Santosa S

Obes Rev. 2016 Jan;17(1):1-17 Authors: Dam V, Sikder T, Santosa S

Article GUID: 26667065
Acute Female Hypogonadism Alters Adipose Tissue Fatty Acid Storage Factors and Chylomicronemia.

Author(s): Santosa S, Bonnes SL, Jensen MD

J Clin Endocrinol Metab. 2016 05;101(5):2089-98 Authors: Santosa S, Bonnes SL, Jensen MD

Article GUID: 27003301
Conjugated linoleic acid mitigates testosterone-related changes in body composition in male guinea pigs.

Author(s): Yang SQ, DeGuire JR, Lavery P, Mak IL, Weiler HA, Santosa S

Nutr Res. 2016 May;36(5):408-17 Authors: Yang SQ, DeGuire JR, Lavery P, Mak IL, Weiler HA, Santosa S

Article GUID: 27101759
Factors associated with adipocyte size reduction after weight loss interventions for overweight and obesity: a systematic review and meta-regression.

Author(s): Murphy J, Moullec G, Santosa S

Metabolism. 2017 Feb;67:31-40 Authors: Murphy J, Moullec G, Santosa S

Article GUID: 28081776
Regional adiposity and markers of inflammation in pre-school age children.

Author(s): Delaney KZ, Vanstone CA, Weiler HA, Santosa S

Sci Rep. 2018 Oct 12;8(1):15204 Authors: Delaney KZ, Vanstone CA, Weiler HA, Santosa S

Article GUID: 30315178
Title:Acetyl-CoA regulation, OXPHOS integrity and leptin level are different in females with different onsets of obesity.
Authors:Tam BT,  Murphy J,  Khor N,  Morais JA,  Santosa S
Link:https://www.ncbi.nlm.nih.gov/pubmed/32808657
DOI:10.1210/endocr/bqaa142
Category:Endocrinology
PMID:32808657
Dept Affiliation: PERFORM
1 Department of Health, Kinesiology, and Applied Physiology, Concordia University, Montreal, Quebec, Canada.
2 Metabolism, Obesity, Nutrition Lab, PERFORM Centre, Concordia University, Montreal, Quebec, Canada.
3 Division of Geriatric Medicine and Research Institute of McGill University Health Centre, Montreal, Quebec, Canada.

Description:

Although childhood-onset obesity (CO) and adult-onset obesity (AO) are known to lead to distinctive clinical manifestations and disease risks, the fundamental differences between them are largely unclear. The aim of the current study is to investigate the fundamental differences between subcutaneous adipose tissue from CO and AO and identify metabolic differences between abdominal (abSAT) and femoral subcutaneous adipose tissues (feSAT). Total and regional body composition was assessed using DXA and computed tomography. Level of acetyl-CoA, NAD+/NADH, acetyl-CoA network genes, mitochondrial complex abundance, H3 acetylation were determined in biopsied abSAT and feSAT. Serum leptin and adiponectin were measured. Our results showed that acetyl-CoA was higher in subcutaneous adipose tissue from subjects with AO compared to CO. Multiple linear regression revealed that ATP citrate lyase was the only main effect affecting the level of acetyl-CoA. Circulating leptin was higher in AO. The increased level of acetyl-CoA was strongly associated with histone H3 acetylation, LEPTIN expression in adipose tissue and circulating leptin in AO. NAD+/NADH was higher in CO, however, mitochondrial complexes abundance, complex II: complex V and complex IV: complex V ratio were lower in CO, reflecting compromised mitochondrial function in adipose tissue from CO. Moreover, we identified differences in the level of acetyl-CoA and NAD+/NADH ratio between abSAT and feSAT, suggesting that these fat depots may possess different metabolic properties. The fundamental difference in the important metabolic intermediate, acetyl-CoA, between CO and AO may help us better understand the development of obesity and the pathogenesis of different obesity-related diseases in human.

PMID: 32808657 [PubMed - as supplied by publisher]