Keyword search (3,448 papers available)


Enzymatic Synthesis of a Fluorogenic Reporter Substrate and the Development of a High-Throughput Assay for Fucosyltransferase VIII Provide a Toolkit to Probe and Inhibit Core Fucosylation.

Author(s): Soroko M, Kwan DH

Biochemistry. 2020 Jun 01;: Authors: Soroko M, Kwan DH

Article GUID: 32441090
Identification of active site residues of chorismate mutase-prephenate dehydrogenase from Escherichia coli.

Author(s): Christendat D, Turnbull J

Identification of active site residues of chorismate mutase-prephenate dehydrogenase from Escherichia coli.

Biochemistry. 1996 Apr 09;35(14):4468-79

Authors: Christendat D, Turnbull J

Abstract
Chemical modification studies...

Article GUID: 8605196
Characterization of active and inactive forms of the phenol hydroxylase stimulatory protein DmpM.

Author(s): Cadieux E, Powlowski J

Biochemistry. 1999 Aug 17;38(33):10714-22 Authors: Cadieux E, Powlowski J

Article GUID: 10451366
S-nitrosation of Ca(2+)-loaded and Ca(2+)-free recombinant calbindin D(28K) from human brain.

Author(s): Tao L, Murphy ME, English AM

Biochemistry. 2002 May 14;41(19):6185-92 Authors: Tao L, Murphy ME, English AM

Article GUID: 11994015
Mechanism of S-nitrosation of recombinant human brain calbindin D28K.

Author(s): Tao L, English AM

Biochemistry. 2003 Mar 25;42(11):3326-34 Authors: Tao L, English AM

Article GUID: 12641465
Protein S-glutathiolation triggered by decomposed S-nitrosoglutathione.

Author(s): Tao L, English AM

Biochemistry. 2004 Apr 06;43(13):4028-38 Authors: Tao L, English AM

Article GUID: 15049710
Mass spectrometric analysis of nitroxyl-mediated protein modification: comparison of products formed with free and protein-based cysteines.

Author(s): Shen B, English AM

Biochemistry. 2005 Oct 25;44(42):14030-44 Authors: Shen B, English AM

Article GUID: 16229492
A shared binding site for NAD+ and coenzyme A in an acetaldehyde dehydrogenase involved in bacterial degradation of aromatic compounds.

Author(s): Lei Y, Pawelek PD, Powlowski J

Biochemistry. 2008 Jul 01;47(26):6870-82 Authors: Lei Y, Pawelek PD, Powlowski J

Article GUID: 18537268
Backbone Flexibility Influences Nucleotide Incorporation by Human Translesion DNA Polymerase η opposite Intrastrand Cross-Linked DNA.

Author(s): O'Flaherty DK, Guengerich FP, Egli M, Wilds CJ

Biochemistry. 2015 Dec 29;54(51):7449-56 Authors: O'Flaherty DK, Guengerich FP, Egli M, Wilds CJ

Article GUID: 26624500
Proton release due to manganese binding and oxidation in modified bacterial reaction centers.

Author(s): Kálmán L, Thielges MC, Williams JC, Allen JP

Biochemistry. 2005 Oct 11;44(40):13266-73 Authors: Kálmán L, Thielges MC, Williams JC, Allen JP

Article GUID: 16201752
Light-induced conformational changes in photosynthetic reaction centers: dielectric relaxation in the vicinity of the dimer.

Author(s): Deshmukh SS, Williams JC, Allen JP, Kálmán L

Biochemistry. 2011 Jan 25;50(3):340-8 Authors: Deshmukh SS, Williams JC, Allen JP, Kálmán L

Article GUID: 21141811
Light-induced conformational changes in photosynthetic reaction centers: redox-regulated proton pathway near the dimer.

Author(s): Deshmukh SS, Williams JC, Allen JP, Kálmán L

Biochemistry. 2011 Apr 26;50(16):3321-31 Authors: Deshmukh SS, Williams JC, Allen JP, Kálmán L

Article GUID: 21410139
Light-induced conformational changes in photosynthetic reaction centers: impact of detergents and lipids on the electronic structure of the primary electron donor.

Author(s): Deshmukh SS, Akhavein H, Williams JC, Allen JP, Kalman L

Biochemistry. 2011 Jun 14;50(23):5249-62 Authors: Deshmukh SS, Akhavein H, Williams JC, Allen JP, Kalman L

Article GUID: 21561160
Title:Backbone Flexibility Influences Nucleotide Incorporation by Human Translesion DNA Polymerase η opposite Intrastrand Cross-Linked DNA.
Authors:O'Flaherty DK,  Guengerich FP,  Egli M,  Wilds CJ
Link:https://www.ncbi.nlm.nih.gov/pubmed/26624500?dopt=Abstract
DOI:10.1021/acs.biochem.5b01078
Category:Biochemistry
PMID:26624500
Dept Affiliation: CHEMBIOCHEM
1 Department of Chemistry and Biochemistry, Concordia University , 7141 Sherbrooke Street West, Montréal, Québec, Canada H4B 1R6.
2 Department of Biochemistry and Center in Molecular Toxicology, Vanderbilt University School of Medicine , Nashville, Tennessee 37232-0146, United States.

Description:

Backbone Flexibility Influences Nucleotide Incorporation by Human Translesion DNA Polymerase ? opposite Intrastrand Cross-Linked DNA.

Biochemistry. 2015 Dec 29;54(51):7449-56

Authors: O'Flaherty DK, Guengerich FP, Egli M, Wilds CJ

Abstract

Intrastrand cross-links (IaCL) connecting two purine nucleobases in DNA pose a challenge to high-fidelity replication in the cell. Various repair pathways or polymerase bypass can cope with these lesions. The influence of the phosphodiester linkage between two neighboring 2'-deoxyguanosine (dG) residues attached through the O(6) atoms by an alkylene linker on bypass with human DNA polymerase ? (hPol ?) was explored in vitro. Steady-state kinetics and mass spectrometric analysis of products from nucleotide incorporation revealed that although hPol ? is capable of bypassing the 3'-dG in a mostly error-free fashion, significant misinsertion was observed for the 5'-dG of the IaCL containing a butylene or heptylene linker. The lack of the phosphodiester linkage triggered an important increase in frameshift adduct formation across the 5'-dG by hPol ?, in comparison to the 5'-dG of IaCL DNA containing the phosphodiester group.

PMID: 26624500 [PubMed - indexed for MEDLINE]