Keyword search (3,448 papers available)


Enzymatic Synthesis of a Fluorogenic Reporter Substrate and the Development of a High-Throughput Assay for Fucosyltransferase VIII Provide a Toolkit to Probe and Inhibit Core Fucosylation.

Author(s): Soroko M, Kwan DH

Biochemistry. 2020 Jun 01;: Authors: Soroko M, Kwan DH

Article GUID: 32441090
Identification of active site residues of chorismate mutase-prephenate dehydrogenase from Escherichia coli.

Author(s): Christendat D, Turnbull J

Identification of active site residues of chorismate mutase-prephenate dehydrogenase from Escherichia coli.

Biochemistry. 1996 Apr 09;35(14):4468-79

Authors: Christendat D, Turnbull J

Abstract
Chemical modification studies...

Article GUID: 8605196
Characterization of active and inactive forms of the phenol hydroxylase stimulatory protein DmpM.

Author(s): Cadieux E, Powlowski J

Biochemistry. 1999 Aug 17;38(33):10714-22 Authors: Cadieux E, Powlowski J

Article GUID: 10451366
S-nitrosation of Ca(2+)-loaded and Ca(2+)-free recombinant calbindin D(28K) from human brain.

Author(s): Tao L, Murphy ME, English AM

Biochemistry. 2002 May 14;41(19):6185-92 Authors: Tao L, Murphy ME, English AM

Article GUID: 11994015
Mechanism of S-nitrosation of recombinant human brain calbindin D28K.

Author(s): Tao L, English AM

Biochemistry. 2003 Mar 25;42(11):3326-34 Authors: Tao L, English AM

Article GUID: 12641465
Protein S-glutathiolation triggered by decomposed S-nitrosoglutathione.

Author(s): Tao L, English AM

Biochemistry. 2004 Apr 06;43(13):4028-38 Authors: Tao L, English AM

Article GUID: 15049710
Mass spectrometric analysis of nitroxyl-mediated protein modification: comparison of products formed with free and protein-based cysteines.

Author(s): Shen B, English AM

Biochemistry. 2005 Oct 25;44(42):14030-44 Authors: Shen B, English AM

Article GUID: 16229492
A shared binding site for NAD+ and coenzyme A in an acetaldehyde dehydrogenase involved in bacterial degradation of aromatic compounds.

Author(s): Lei Y, Pawelek PD, Powlowski J

Biochemistry. 2008 Jul 01;47(26):6870-82 Authors: Lei Y, Pawelek PD, Powlowski J

Article GUID: 18537268
Backbone Flexibility Influences Nucleotide Incorporation by Human Translesion DNA Polymerase η opposite Intrastrand Cross-Linked DNA.

Author(s): O'Flaherty DK, Guengerich FP, Egli M, Wilds CJ

Biochemistry. 2015 Dec 29;54(51):7449-56 Authors: O'Flaherty DK, Guengerich FP, Egli M, Wilds CJ

Article GUID: 26624500
Proton release due to manganese binding and oxidation in modified bacterial reaction centers.

Author(s): Kálmán L, Thielges MC, Williams JC, Allen JP

Biochemistry. 2005 Oct 11;44(40):13266-73 Authors: Kálmán L, Thielges MC, Williams JC, Allen JP

Article GUID: 16201752
Light-induced conformational changes in photosynthetic reaction centers: dielectric relaxation in the vicinity of the dimer.

Author(s): Deshmukh SS, Williams JC, Allen JP, Kálmán L

Biochemistry. 2011 Jan 25;50(3):340-8 Authors: Deshmukh SS, Williams JC, Allen JP, Kálmán L

Article GUID: 21141811
Light-induced conformational changes in photosynthetic reaction centers: redox-regulated proton pathway near the dimer.

Author(s): Deshmukh SS, Williams JC, Allen JP, Kálmán L

Biochemistry. 2011 Apr 26;50(16):3321-31 Authors: Deshmukh SS, Williams JC, Allen JP, Kálmán L

Article GUID: 21410139
Light-induced conformational changes in photosynthetic reaction centers: impact of detergents and lipids on the electronic structure of the primary electron donor.

Author(s): Deshmukh SS, Akhavein H, Williams JC, Allen JP, Kalman L

Biochemistry. 2011 Jun 14;50(23):5249-62 Authors: Deshmukh SS, Akhavein H, Williams JC, Allen JP, Kalman L

Article GUID: 21561160
Title:Characterization of active and inactive forms of the phenol hydroxylase stimulatory protein DmpM.
Authors:Cadieux E,  Powlowski J
Link:https://www.ncbi.nlm.nih.gov/pubmed/10451366?dopt=Abstract
DOI:10.1021/bi990835q
Category:Biochemistry
PMID:10451366
Dept Affiliation: CHEMBIOCHEM
1 Department of Chemistry and Biochemistry, Concordia University, Montreal, Quebec, Canada.

Description:

Characterization of active and inactive forms of the phenol hydroxylase stimulatory protein DmpM.

Biochemistry. 1999 Aug 17;38(33):10714-22

Authors: Cadieux E, Powlowski J

Abstract

The stimulatory protein DmpM of phenol hydroxylase from methylphenol-degrading Pseudomonas sp. strain CF600 has been found to exist in two forms. DmpM purified from the native strain was mostly active in stimulating phenol hydroxylase activity, whereas an inactive form accumulated in a recombinant strain. Both forms exhibited a molecular mass of 10 361.3 +/- 1.3 Da by electrospray mass spectrometry, but nondenaturing gel filtration showed molecular masses of 31 600 Da for the inactive form and 11 500 Da for the active form. Cross-linking and sedimentation velocity results were consistent with the inactive form being a dimer. Partial thermal or chemical denaturation, or treatment with trifluoroethanol, readily activated dimeric DmpM. A combination of circular dichroism and fluorescence spectroscopies, activity assays, and native and urea gel electrophoresis were used to further characterize reactivation with urea. These results showed that dissociation of the dimeric form of DmpM precedes denaturation at low protein concentrations and results in activation. The same concentration of urea that effects dissociation also converts the monomeric form to a different conformation.

PMID: 10451366 [PubMed - indexed for MEDLINE]