Keyword search (3,448 papers available)


Enzymatic Synthesis of a Fluorogenic Reporter Substrate and the Development of a High-Throughput Assay for Fucosyltransferase VIII Provide a Toolkit to Probe and Inhibit Core Fucosylation.

Author(s): Soroko M, Kwan DH

Biochemistry. 2020 Jun 01;: Authors: Soroko M, Kwan DH

Article GUID: 32441090
Identification of active site residues of chorismate mutase-prephenate dehydrogenase from Escherichia coli.

Author(s): Christendat D, Turnbull J

Identification of active site residues of chorismate mutase-prephenate dehydrogenase from Escherichia coli.

Biochemistry. 1996 Apr 09;35(14):4468-79

Authors: Christendat D, Turnbull J

Abstract
Chemical modification studies...

Article GUID: 8605196
Characterization of active and inactive forms of the phenol hydroxylase stimulatory protein DmpM.

Author(s): Cadieux E, Powlowski J

Biochemistry. 1999 Aug 17;38(33):10714-22 Authors: Cadieux E, Powlowski J

Article GUID: 10451366
S-nitrosation of Ca(2+)-loaded and Ca(2+)-free recombinant calbindin D(28K) from human brain.

Author(s): Tao L, Murphy ME, English AM

Biochemistry. 2002 May 14;41(19):6185-92 Authors: Tao L, Murphy ME, English AM

Article GUID: 11994015
Mechanism of S-nitrosation of recombinant human brain calbindin D28K.

Author(s): Tao L, English AM

Biochemistry. 2003 Mar 25;42(11):3326-34 Authors: Tao L, English AM

Article GUID: 12641465
Protein S-glutathiolation triggered by decomposed S-nitrosoglutathione.

Author(s): Tao L, English AM

Biochemistry. 2004 Apr 06;43(13):4028-38 Authors: Tao L, English AM

Article GUID: 15049710
Mass spectrometric analysis of nitroxyl-mediated protein modification: comparison of products formed with free and protein-based cysteines.

Author(s): Shen B, English AM

Biochemistry. 2005 Oct 25;44(42):14030-44 Authors: Shen B, English AM

Article GUID: 16229492
A shared binding site for NAD+ and coenzyme A in an acetaldehyde dehydrogenase involved in bacterial degradation of aromatic compounds.

Author(s): Lei Y, Pawelek PD, Powlowski J

Biochemistry. 2008 Jul 01;47(26):6870-82 Authors: Lei Y, Pawelek PD, Powlowski J

Article GUID: 18537268
Backbone Flexibility Influences Nucleotide Incorporation by Human Translesion DNA Polymerase η opposite Intrastrand Cross-Linked DNA.

Author(s): O'Flaherty DK, Guengerich FP, Egli M, Wilds CJ

Biochemistry. 2015 Dec 29;54(51):7449-56 Authors: O'Flaherty DK, Guengerich FP, Egli M, Wilds CJ

Article GUID: 26624500
Proton release due to manganese binding and oxidation in modified bacterial reaction centers.

Author(s): Kálmán L, Thielges MC, Williams JC, Allen JP

Biochemistry. 2005 Oct 11;44(40):13266-73 Authors: Kálmán L, Thielges MC, Williams JC, Allen JP

Article GUID: 16201752
Light-induced conformational changes in photosynthetic reaction centers: dielectric relaxation in the vicinity of the dimer.

Author(s): Deshmukh SS, Williams JC, Allen JP, Kálmán L

Biochemistry. 2011 Jan 25;50(3):340-8 Authors: Deshmukh SS, Williams JC, Allen JP, Kálmán L

Article GUID: 21141811
Light-induced conformational changes in photosynthetic reaction centers: redox-regulated proton pathway near the dimer.

Author(s): Deshmukh SS, Williams JC, Allen JP, Kálmán L

Biochemistry. 2011 Apr 26;50(16):3321-31 Authors: Deshmukh SS, Williams JC, Allen JP, Kálmán L

Article GUID: 21410139
Light-induced conformational changes in photosynthetic reaction centers: impact of detergents and lipids on the electronic structure of the primary electron donor.

Author(s): Deshmukh SS, Akhavein H, Williams JC, Allen JP, Kalman L

Biochemistry. 2011 Jun 14;50(23):5249-62 Authors: Deshmukh SS, Akhavein H, Williams JC, Allen JP, Kalman L

Article GUID: 21561160
Title:Protein S-glutathiolation triggered by decomposed S-nitrosoglutathione.
Authors:Tao L,  English AM
Link:https://www.ncbi.nlm.nih.gov/pubmed/15049710?dopt=Abstract
DOI:10.1021/bi035924o
Category:Biochemistry
PMID:15049710
Dept Affiliation: CHEMBIOCHEM
1 Department of Chemistry and Biochemistry, Concordia University, 7141 Sherbrooke Street West, Montreal, Quebec, Canada H4B 1R6.

Description:

Protein S-glutathiolation triggered by decomposed S-nitrosoglutathione.

Biochemistry. 2004 Apr 06;43(13):4028-38

Authors: Tao L, English AM

Abstract

Recombinant human brain calbindin D(28K) (rHCaBP), human Cu,Zn-superoxide dismutase (HCuZnSOD), rabbit muscle glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and bovine serum albumin (BSA) were found to be S-glutathiolated in decomposed S-nitrosoglutathione (GSNO) solutions. Tryptic or Glu-C digestion and MALDI-TOF MS analyses of the digests are consistent with S-thiolation of Cys111 and Cys187 of HCuZnSOD and rHCaBP, respectively, upon exposure to decomposed GSNO. GAPDH activity analysis reveals that S-glutathiolation most likely occurs on the active site Cys149, and the single free Cys34 is assumed to be the site of S-glutathiolation in BSA. The yields of S-glutathiolation of rHCaBP, GAPDH, and BSA were much higher than those of HCuZnSOD. The latter is limited by the accessibility of Cys111 to the glutathiolating reagent in the HCuZnSOD dimer. Unlike decomposed GSNO, fresh GSNO, reduced glutathione (GSH), and oxidized glutathione (GSSG) are not efficient S-glutathiolating agents for the proteins examined here. On the basis of analysis by mass spectrometry and UV-visible absorption, GSNO decomposition in the dark at room temperature yields glutathione disulfide S-oxide [GS(O)SG], glutathione disulfide S-dioxide (GSO(2)SG), and GSSG as products. GS(O)SG is the efficient protein S-glutathiolating agent in GSNO solutions, not GSNO, which does not carry out efficient S-glutathiolation of rHCaBP, HCuZnSOD, or GAPDH in vitro. A hydrolysis pathway yielding GSOH and nitroxyl (HNO/NO(-)) as intermediates is proposed for GSNO decomposition in the dark. This is based on inhibition of GSNO breakdown by dimedone, a reagent specific for sulfenic acids, and on nitroxyl scavenging by metmyoglobin. The results presented here are contrary to numerous reports of protein S-thiolation by low-molecular weight S-nitrosothiols.

PMID: 15049710 [PubMed - indexed for MEDLINE]